MMSN@Gem的构建及其抑制人膀胱癌细胞系BIU-87增殖和促凋亡

doi:10.16352/j.issn.1001-6325.2025.06.0777

基础医学与临床 ›› 2025, Vol. 45 ›› Issue (6): 777-785.doi: 10.16352/j.issn.1001-6325.2025.06.0777

MMSN@Gem的构建及其抑制人膀胱癌细胞系BIU-87增殖和促凋亡

王大亚^*, 李志家, 赵德威, 陈熙猛

温州市中心医院泌尿外科,浙江温州 325000

收稿日期:2024-06-13 修回日期:2024-11-22 出版日期:2025-06-05 发布日期:2025-05-26
通讯作者: ^*22154468@qq.com
基金资助:
温州市基础科研项目(Y20210946)

Construction of MMSN@Gem and its inhibition of proliferation and promotion of apoptosis in human bladder cancer cell line BIU-87

WANG Daya^*, LI Zhijia, ZHAO Dewei, CHEN Ximeng

Department of Urology, Wenzhou Central Hospital,Wenzhou 325000, China

Received:2024-06-13 Revised:2024-11-22 Online:2025-06-05 Published:2025-05-26

摘要/Abstract

摘要： 目的制备携吉西他滨多功能介孔硅基纳米载药体系(MMSN@Gem),并研究其对膀胱癌细胞BIU-87的影响。方法通过改良方法制备多功能介孔硅基纳米载药体系。利用透射电子显微镜、高效液相色谱仪和热成像仪对MMSN@Gem的形貌、载药及光热性能进行表征。采用体外实验检测MMSN@Gem对人膀胱癌细胞BIU-87的影响。结果 MMSN@Gem形貌为规则圆形,平均粒径为(102.48±1.03)nm,载药量为25.04%±0.17%,平均zeta电位为(-24.84±0.07)mV,光热转化效率为40.7%,在近红外照射下显著提升Gem的释放能力。体外研究表明MMSN@Gem显著抑制BIU-87细胞活性,诱导BIU-87细胞凋亡,降低了细胞迁移和侵袭能力,并增强BIU-87细胞对其摄取的能力。结论 MMSN@Gem具有优异的光热性能,可增强细胞摄取效率,且能够抑制BIU-87细胞的增殖和侵袭迁移,促进凋亡,为临床治疗膀胱肿瘤提供了一种具有前景的药物递送系统。

关键词: 吉西他滨, 纳米载药体系, 膀胱肿瘤, 介孔硅基

Abstract: Objective To prepare a multifunctional mesoporous silica-based nanocarrier system(MMSN@Gem) with gemcitabine and investigate its effect on bladder cancer cells BIU-87. Methods The multifunctional mesoporous silica-based nano drug-carrying system was prepared by a modified method. Transmission electron microscopy, high-performance liquid chromatography, and thermal imaging were used to characterize the morphology, drug-carrying and photothermal properties of MMSN@Gem. The effect of MMSN@Gem on BIU-87 bladder cancer cells was detected by in vitro experiments. Results MMSN@Gem exhibited a well-defined spherical morphology with an average particle size of(102.48±1.03)nm with a drug loading capacity of 25.04%±0.17%, and an average zeta potential of(-24.84±0.07)mV. The photothermal conversion efficiency was 40.7% which significantly enhanced the release of Gem under near-infrared irradiation. In vitro studies showed that MMSN@Gem significantly inhibited BIU-87 cell activity, induced apoptosis of BIU-87 cells, reduced migration and invasion ability, and enhanced its uptake by BIU-87 cells. Conclusions MMSN@Gem exhibits excellent photothermal properties, enhances cellular uptake efficiency, inhibits the proliferation and migration of BIU-87 cells, and promotes apoptosis, providing a promising drug delivery system for the clinical treatment of bladder cancer.

Key words: gemcitabine, nano co-loading system, bladder tumour, mesoporous silica

中图分类号:

R945

王大亚, 李志家, 赵德威, 陈熙猛. MMSN@Gem的构建及其抑制人膀胱癌细胞系BIU-87增殖和促凋亡[J]. 基础医学与临床, 2025, 45(6): 777-785.

WANG Daya, LI Zhijia, ZHAO Dewei, CHEN Ximeng. Construction of MMSN@Gem and its inhibition of proliferation and promotion of apoptosis in human bladder cancer cell line BIU-87[J]. Basic & Clinical Medicine, 2025, 45(6): 777-785.

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MMSN@Gem的构建及其抑制人膀胱癌细胞系BIU-87增殖和促凋亡

Construction of MMSN@Gem and its inhibition of proliferation and promotion of apoptosis in human bladder cancer cell line BIU-87

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