基于探针的无标记表面增强拉曼光谱(SERS)用于鉴别乳腺癌患者的血清代谢谱

doi:10.16352/j.issn.1001-6325.2025.05.0651

基础医学与临床 ›› 2025, Vol. 45 ›› Issue (5): 651-657.doi: 10.16352/j.issn.1001-6325.2025.05.0651

基于探针的无标记表面增强拉曼光谱(SERS)用于鉴别乳腺癌患者的血清代谢谱

王盟¹, 沈学静², 刘佳², 尚璐璐³, 张沫^1*

1.中国医学科学院基础医学研究所北京协和医学院基础学院生物物理及结构生物学系,北京 100005;
2.钢研纳克检测技术股份有限公司纳克研究院,北京 100081;
3.中国人民解放军河南省军区郑州第三离职干部休养所综合门诊部,河南郑州 450006

收稿日期:2024-10-24 修回日期:2024-12-31 出版日期:2025-05-05 发布日期:2025-04-23
通讯作者: ^* zhangmo@ibms.pumc.edu.cn
基金资助:
北京市自然科学基金海淀原始创新联合基金(L232024);中国医学科学院医学与健康科技创新工程(2023-I2M-QJ-014)

Probe-based label-free SERS for identification of breast cancer-induced serum metabolic profiles in patients

WANG Meng¹, SHEN Xuejing², LIU Jia², SHANG Lulu³, ZHANG Mo^1*

1. Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences CAMS, School of Basic Medicine PUMC, Beijing 100005;
2. NCS Institute, NCS Testing Technology Company Limited, Beijing 100081;
3. Comprehensive Outpatient Department, the Third Retired Cadres Retreat of Henan Military Region of the Chinese People′s Liberation Army, Zhengzhou 450006, China

Received:2024-10-24 Revised:2024-12-31 Online:2025-05-05 Published:2025-04-23
Contact: ^* zhangmo@ibms.pumc.edu.cn

摘要/Abstract

摘要： 目的应用无标记的表面增强拉曼光谱(SERS)检测血清代谢物,快速区分乳腺癌患者和健康受试者的代谢谱。方法合成等离激元纳米材料作为SERS的探针,检测拉曼报告分子,评估基于该探针的SERS分析能力。收集乳腺癌患者和健康受试者的血清样品,用甲醇沉淀蛋白质并去除后得到血清代谢物。基于探针的SERS分析患者的血清代谢物,探究乳腺癌患者的代谢谱变化。结果合成了SERS探针并进行了表征;建立了基于SERS探针的分析方法,实现了4个数量级的线性范围(LR),检测限(LOD)可达10 nmol/L;分析了5例乳腺癌患者和5名健康受试者的血清代谢物的拉曼光谱(RS),研究了代谢物变化的差异(P＜0.001)。结论本研究通过基于探针的SERS方法筛选出乳腺癌患者血清代谢物的分子谱图差异,有助于解释乳腺癌引起的代谢改变,为乳腺癌的筛查、快速检测提供新方法。

关键词: 乳腺癌, 探针, 快速检测, 表面增强拉曼光谱, 代谢谱

Abstract: Objective To detect Serum metabolites with label-free surface-enhanced Raman spectroscopy (SERS) for quickly distinguishing the metabolic profiles of breast cancer patients and healthy subjects. Methods A kind of Plasma nano-material was synthesized as a probe for SERS, which has also been used to detect Raman reporter molecules to assess its detection capability. Serum samples from breast cancer patients and healthy subjects were collected and the proteins were precipitated with methanol and removed to collect serum metabolites. Probe-based SERS was used to analyze the serum metabolites of patients and explore the changes in the metabolic profiles of breast cancer patients. Results The SERS probe was synthesized and validated. An analytical method based on SERS probe was established, which achieved a linear range (LR) of 4 orders of magnitude and a limit of detection (LOD) up to 10 nmol/L. Raman spectra of serum metabolites from 5 breast cancer patients and 5 healthy subjects were analyzed to study differences in metabolite changes. Conclusions In this study, the molecular spectrum differences of serum metabolites in breast cancer patients were screened by probe-based SERS method, which provides a technology support research on the metabolic changes caused by breast cancer so potentially provide a new method for fast breast cancer screening.

Key words: breast cancer, probe, rapid detection, surface-enhanced Raman spectroscopy, metabolic profiles

中图分类号:

王盟, 沈学静, 刘佳, 尚璐璐, 张沫. 基于探针的无标记表面增强拉曼光谱(SERS)用于鉴别乳腺癌患者的血清代谢谱[J]. 基础医学与临床, 2025, 45(5): 651-657.

WANG Meng, SHEN Xuejing, LIU Jia, SHANG Lulu, ZHANG Mo. Probe-based label-free SERS for identification of breast cancer-induced serum metabolic profiles in patients[J]. Basic & Clinical Medicine, 2025, 45(5): 651-657.

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基于探针的无标记表面增强拉曼光谱(SERS)用于鉴别乳腺癌患者的血清代谢谱

Probe-based label-free SERS for identification of breast cancer-induced serum metabolic profiles in patients

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