乌司他丁联合生长抑素减轻急性胰腺炎大鼠肠道损伤

doi:10.16352/j.issn.1001-6325.2025.02.0203

基础医学与临床 ›› 2025, Vol. 45 ›› Issue (2): 203-209.doi: 10.16352/j.issn.1001-6325.2025.02.0203

乌司他丁联合生长抑素减轻急性胰腺炎大鼠肠道损伤

靳高超^1*, 邢盼盼², 王玉³, 刘波³

邢台市人民医院 1.胰胆外科;
2.消化内科;
3.重症医学科,河北邢台 054000

收稿日期:2024-05-13 修回日期:2024-07-19 出版日期:2025-02-05 发布日期:2025-01-17
通讯作者: ^*jiaxiayv36991@163.com
基金资助:
2023年邢台市市级科技计划自筹经费项目(2023ZC062)

Combination of ulinastatin and somatostatin attenuates intestinal injury in rats with acute pancreatitis

JIN Gaochao^1*, XING Panpan², WANG Yu³, LIU Bo³

1. Department of Pancreaticobiliary Surgery;
2. Department of Gastroenterology;
3. Department of Critical Care Medicine, Xingtai People's Hospital, Xingtai 054000, China

Received:2024-05-13 Revised:2024-07-19 Online:2025-02-05 Published:2025-01-17
Contact: ^*jiaxiayv36991@163.com

摘要/Abstract

摘要： 目的探讨乌司他丁(UTI)与生长抑素(SOM)对急性胰腺炎(AP)大鼠肠道损伤的影响及可能的作用机制。方法将大鼠随机分为假手术(sham)组、AP组、UTI组、UIT+SOM组和UIT+SOM+JAK2激活剂(CA1)组,分别建立AP大鼠模型立,15只/组。结束给药12 h后,检测大鼠肠道通透性以及血清生化指标、炎性因子水平;苏木精-伊红(HE)染色观察胰腺和肠道组织的病理变化;TUNEL法检测肠道组织细胞凋亡;Western blot检测Janus激酶2(JAK2)信号转导与转录激活因子3(STAT3)信号通路蛋白表达。结果与假手术组比较,AP组大鼠胰腺组织炎性细胞浸润、水肿和出血明显,肠组织中肠黏膜出现炎性细胞浸润、绒毛不规则、肠上皮细胞脱落和坏死,肠道通透性、血清淀粉酶(AMY)和脂肪酶(LIPA)活性、二胺氧化酶(DAO)活性、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平、胰腺和肠道病理组织学评分、肠组织细胞凋亡率及p-JAK2/JAK2、p-STAT3/STAT3比值均升高(P＜0.05);与AP组比较,UTI组、UIT+SOM组大鼠胰腺和肠道组织损伤减轻,炎性细胞浸润减少,肠道通透性、血清AMY、LIPA活性、DAO活性、TNF-α、IL-6水平、胰腺和肠道病理组织学评分、肠组织细胞凋亡率及p-JAK2/JAK2、p-STAT3/STAT3比值均降低(P＜0.05);UIT+SOM+CA1组大鼠胰腺和肠道组织损伤加重,上述指标趋势均与UIT+SOM组相反(P＜0.05)。结论 UTI联合SOM可减轻AP大鼠的肠道损伤,可能与抑制JAK2/STAT3信号通路有关。

关键词: 乌司他丁, 生长抑素, 急性胰腺炎, 肠道损伤, Janus激酶2/信号转导与转录激活因子3信号通路(JAK2/STAT3)

Abstract: Objective To investigate the effects of ulinastatin (UTI) combined with somatostatin (SOM) on intestinal damage in acute pancreatitis (AP) rats and the possible mechanisms of action. Methods The rats were randomly divided into sham-operated (sham) group, AP group, UTI group, UIT+SOM group and UIT+SOM+JAK2 activator (CA1) group, 15 rats/group, respectively. Twelve hours after administration, the intestinal permeability, serum biochemical indicators, and inflammatory factor of rats were detected. HE staining was applied to observe the pathological changes in pancreatic and intestinal tissues. TUNEL method was applied to detect apoptosis in intestinal tissue. Western blot was applied to detect the expression of JAK2/STAT3 signaling pathway proteins. Results Compared with the sham group, the pancreatic tissue of rats in the AP group showed obvious inflammatory cell infiltration, edema and bleeding, while the intestinal mucosa showed inflammatory cell infiltration, irregular villi, shedding and necrosis of intestinal epithelial cells in the intestinal tissue. The intestinal permeability, serum amylase (AMY), lipase(LIPA) activities, diamine oxidase (DAO) activities, tumor necrosis factor α(TNF-α), interleukin-6(IL-6) level, pancreatic and intestinal histopathological scores, intestinal tissue cell apoptosis rate, and p-JAK2/JAK2, p-STAT3/STAT3 ratio all significantly increased (P＜0.05). Compared with the AP group, the pancreatic and intestinal tissue injury of rats in the UTI group and UIT+SOM group was reduced, and inflammatory cell infiltration was reduced. The intestinal permeability, serum AMY, LIPA activities, DAO activities, TNF-α, IL-6 level, pancreatic and intestinal histopathological scores, intestinal tissue cell apoptosis rate and p-JAK2/JAK2, p-STAT3/STAT3 ratio were all decreased (P＜0.05). The pancreatic and intestinal tissue injury of rats in the UIT+SOM+CA1 group were more severe, and the trends of the above indicators were opposite to those found in UIT+SOM group (P＜0.05). Conclusions The combination of UTI and SOM attenuated intestinal injury in AP rats, and potential mechanism may involve in the inhibition of the JAK2/STAT3 signaling pathway.

Key words: ulinastatin, somatostatin, acute pancreatitis, intestinal injury, JAK2/STAT3

中图分类号:

R576

靳高超, 邢盼盼, 王玉, 刘波. 乌司他丁联合生长抑素减轻急性胰腺炎大鼠肠道损伤[J]. 基础医学与临床, 2025, 45(2): 203-209.

JIN Gaochao, XING Panpan, WANG Yu, LIU Bo. Combination of ulinastatin and somatostatin attenuates intestinal injury in rats with acute pancreatitis[J]. Basic & Clinical Medicine, 2025, 45(2): 203-209.

参考文献 13

[1]	Liu LW, Xie Y, Li GQ, et al. Gut microbiota-derived nicotinamide mononucleotide alleviates acute pancreatitis by activating pancreatic SIRT3 signalling[J]. Br J Pharmacol, 2023, 180:647-666.
[2]	Wei B, Wu Q, Yang X, et al. Effect of TRAF6 in acute pancreatitis-induced intestinal barrier injury via TLR4/NF-κB signal pathway[J]. Tissue Cell, 2022, 76:101792. doi: 10.1016/j.tice.2022.101792.
[3]	Yang X, Guo N. Ulinastatin ameliorates podocyte ferroptosis via regulating miR-144-3p/SLC7A11 axis in acute kidney injury[J]. In Vitro Cell Dev Biol Anim, 2023, 59:697-705.
[4]	Liguz-Lecznar M, Dobrzanski G, Kossut M. Somatostatin and somatostatin-containing interneurons-from plasticity to pathology[J]. Biomolecules, 2022, 12:312-329.
[5]	朱理珂, 黄鹏, 梁晴晴. 乌司他丁、生长抑素联合在急性胰腺炎中的应用价值及对肠黏膜屏障功能的影响[J]. 中国医学工程, 2023, 31:120-124.
[6]	Chen F, Xu Y, Wang Z. Ulinastatin combined with somatostatin enhances disease control and modulates serum inflammatory factors in patients with severe pancreatitis[J]. Am J Transl Res, 2023, 15:5797-5807.
[7]	Yang X, Geng H, You L, et al. Rhein protects against severe acute pancreatitis in vitro and in vivo by regulating the JAK2/STAT3 pathway[J]. Front Pharmacol, 2022, 13:778221-778233.
[8]	唐祖鑫, 左雪虹, 付娟, 等. 柴黄清胰活血颗粒对重症急性胰腺炎模型大鼠JAK2/STAT3信号通路的影响[J]. 中药新药与临床药理, 2022, 33:1055-1062.
[9]	Jin W, Shen Y. Da-Cheng-Qi decoction alleviates intestinal injury in rats with severe acute pancreatitis by inhibiting the JAK2-STAT3 signaling pathway[J]. Evid Based Complement Alternat Med, 2019, 2019:3909468-3909480.
[10]	黄伟,孔丽君,叶亚群,等.AMPK激活剂减轻重症急性胰腺炎模型大鼠胰腺损伤[J].基础医学与临床,2021,41:1606-1611.
[11]	Su SY, Tang QQ. Altered intestinal microflora and barrier injury in severe acute pancreatitis can be changed by zinc[J]. Int J Med Sci, 2021, 18:3050-3058.
[12]	Su YR, Hong YP, Mei FC, et al. High-fat diet aggravates the intestinal barrier injury via TLR4-RIP3 pathway in a rat model of severe acute pancreatitis[J]. Mediators Inflamm, 2019, 2019:2512687-2512700.
[13]	Yang S, Song Y, Wang Q, et al. Daphnetin ameliorates acute lung injury in mice with severe acute pancreatitis by inhibiting the JAK2-STAT3 pathway[J]. Sci Rep, 2021, 11:11491-11502.

编辑推荐

Metrics

阅读次数

全文

HTML			PDF

最新录用	在线预览	正式出版	最新录用	在线预览	正式出版
0	0	0	0	0	8

	来源	本网站

	次数	8
	比例	100%

摘要

最新录用	在线预览	正式出版

0	0	48

	来源	本网站

	次数	48
	比例	100%

乌司他丁联合生长抑素减轻急性胰腺炎大鼠肠道损伤

Combination of ulinastatin and somatostatin attenuates intestinal injury in rats with acute pancreatitis

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献 13

相关文章 12

编辑推荐

Metrics

本文评价

[1]	殷强, 汪磊, 李童浩. 牛磺熊去氧胆酸减轻重症急性胰腺炎诱导的模型大鼠肝损伤[J]. 基础医学与临床, 2024, 44(9): 1249-1255.
[2]	王宇, 童安莉, 周玥, 张文倩, 崔云英, 景红丽, 李玉秀. ^99mTc-HYNIC-TOC显像和¹³¹I-MIBG显像在嗜铬细胞瘤和副神经节瘤中的诊断价值[J]. 基础医学与临床, 2024, 44(3): 374-378.
[3]	韩崇屹, 王久吉, 朱丽美, 刘前, 孙建利. 激活PI3K/AKT/mTOR通路降低急性胰腺炎模型大鼠炎性反应[J]. 基础医学与临床, 2024, 44(11): 1563-1568.
[4]	苏拾香, 王语阳, 覃宗帅, 黄桂香, 徐键, 岑兰英, 覃月秋. miR-142-3p通过调控Hmgb1抑制雨蛙素诱导的大鼠胰腺外分泌细胞系AR42J凋亡[J]. 基础医学与临床, 2024, 44(1): 23-30.
[5]	景光旭, 王张鹏, 刘忠钰, 吕双, 孙红玉. 低氧预处理胎盘间充质干细胞减轻重症急性胰腺炎模型小鼠胰腺组织损伤[J]. 基础医学与临床, 2023, 43(9): 1346-1352.
[6]	谷文浩, 郭飞霞, 徐宇鹏, 陆馨雨, 胡青青. 淫羊藿苷改善重症急性胰腺炎模型大鼠肺损伤[J]. 基础医学与临床, 2022, 42(9): 1400-1405.
[7]	韦碧薇, 龚雅慧, 苏州, 杨慧莹, 覃蒙斌, 梁志海. miR-125b参与TRAF6影响的人胰腺导管上皮屏障作用[J]. 基础医学与临床, 2021, 41(9): 1272-1276.
[8]	黄伟, 孔丽君, 叶亚群, 王宏伟. AMPK激活剂减轻重症急性胰腺炎模型大鼠胰腺损伤[J]. 基础医学与临床, 2021, 41(11): 1606-1611.
[9]	何锐, 朱烨柯, 滕文彬, 单跃, 易声华, 祝胜美, 李玉红. 甘油可能通过水通道蛋白3减轻脓毒症小鼠肠黏膜屏障损伤[J]. 基础医学与临床, 2020, 40(5): 655-661.
[10]	陈振宇, 黄启林, 孙红玉, 刘立业, 文艺. 腹腔穿刺引流对早期重症急性胰腺炎大鼠肝脏TLR4表达的影响[J]. 基础医学与临床, 2020, 40(10): 1342-1347.
[11]	唐帅徐仲煌杨宏任丽英钱文伟翁习生黄宇光. 乌司他丁减轻双侧全膝关节置换术后患者炎性反应和急慢性疼痛[J]. 基础医学与临床, 2014, 34(4): 514-518.
[12]	薛小军涂小煌陈少全. 间充质干细胞治疗急性胰腺炎的研究进展[J]. 基础医学与临床, 2010, 30(10): 1109-1111.