关键词: 妊娠期糖尿病, 微RNA, miR-483-3p, 诊断

Abstract: Objective To investigate the expression and clinical significance of miR-483-3p in patients with gestational diabetes mellitus (GDM). Methods A total of 100 GDM patients with 24-32 weeks of gestation who underwent routine obstetric examination in Anqiu People′s Hospital were selected as the normal glucose tolerance (NGT) group, and a total of 98 healthy pregnant women of the same age of 24-32 gestational weeks were selected as the NGT group. The expression level of miR-483-3p was detected by RT-qPCR, the diagnostic value of miR-483-3p in GDM was analyzed by receiver operating characteristic (ROC) curve, the correlation between the expression levels of miR-483-3p and clinical indicators in the GDM group was analyzed by chi-square test, and the risk factors of GDM were analyzed by Logistic regression. Dual luciferase reporter gene assay was used to verify the targeting relationship between miR-483-3p and autophagy-related protein 7(ATG7). CCK8, flow cytometry and Transwell assays were used to detect the proliferation, apoptosis, and migration and invasion ability of cells, respectively. Results The expression level of miR-483-3p in the serum of patients in the GDM group was significantly higher than that in the NGT group. The expression of miR-483-3p had diagnostic value for GDM. Pre-pregnancy BMI, FBG, FINS, HOMA-IR and TG were significantly correlated with serum miR-483-3p expression. In addition, BMI and TG before pregnancy were risk factors leading to GDM. miR-483-3p regulated HG-treated HTR-8/SVneo cell proliferation, apoptosis, migration and invasion by targeting at ATG7. Conclusions miR-483-3p is involved in the disease progression of GDM and is a potential biomarker of GDM diagnosis.

Key words: gestational diabetes mellitus, microRNA, miR-483-3p, diagnosis