流体剪切力通过激活Piezo 1蛋白促进肾小球内皮细胞凋亡

doi:10.16352/j.issn.1001-6325.2024.09.1236

基础医学与临床 ›› 2024, Vol. 44 ›› Issue (9): 1236-1242.doi: 10.16352/j.issn.1001-6325.2024.09.1236

流体剪切力通过激活Piezo 1蛋白促进肾小球内皮细胞凋亡

罗梦琳^1,2,3, 郑峰⁴, 季欣瑶², 祁彩虹², 肖雨珩², 牛长春^1,2*

1.西南医科大学附属医院检验科,四川泸州 646099;
2.重庆市人民医院检验科,重庆 401121;
青海省人民医院 3.检验科;4.骨科,青海西宁 810007

收稿日期:2023-11-09 修回日期:2024-05-08 出版日期:2024-09-05 发布日期:2024-08-30
通讯作者: ^*bright_star2000@163.com
基金资助:
青海省卫生健康委员会重点课题(2022-wjzd-02)

Fluid shear promotes apoptosis of glomerular endothelial cells by activation of Piezo 1

LUO Menglin^1,2,3, ZHENG Feng⁴, JI Xinyao², QI Caihong², XIAO Yuheng², NIU Changchun^1,2*

1. Department of Laboratory, the Affiliated Hospital of Southwest Medical University, Luzhou 646099;
2. Department of Laboratory, Chongqing People′s Hospital, Chongqing 401121;
3. Department of Laboratory; 4. Department of Orthopedics, Qinghai Provincial People′s Hospital, Xining 810007, China

Received:2023-11-09 Revised:2024-05-08 Online:2024-09-05 Published:2024-08-30
Contact: ^*bright_star2000@163.com

摘要/Abstract

摘要： 目的探讨流体剪切力(FS)对肾小球内皮细胞(GECs)凋亡的影响及Piezo 1蛋白在其中的作用。方法对SD大鼠肾小球内皮细胞进行培养,使用Flexcell-T5000拉力仪模拟流体剪切力刺激,使用流式细胞测量技术检测细胞凋亡水平,通过免疫荧光染色试验检测Piezo 1蛋白在GECs中的表达水平。使用Ca²⁺指示剂(Cal-590 AM)观察流体剪切力对Piezo 1通道的激活作用。使用化学激动剂Yoda1、抑制剂GsMtx 4及慢病毒Lv-shPiezo 1调控Piezo 1蛋白功能或表达后,观察Piezo 1对GECs凋亡的影响。结果与对照组相比,剪切力组细胞凋亡率增高(P＜0.05),且随着流体剪切力强度升高,细胞凋亡率增高;Piezo 1在GECs中普遍表达,且流体剪切力可以激活Piezo 1通道并增强其表达;激动剂Yoda 1可以促进GECs的凋亡,抑制剂GsMtx 4可以抑制流体剪切力诱导的凋亡;Lv-shPiezo 1敲低GECs中Piezo 1的表达,且敲低组GECs的凋亡率较对照组及Lv-Ctrl组降低(P＜0.05)。结论流体剪切力通过激活Piezo 1蛋白及增强其表达促进GECs的凋亡。

关键词: 流体剪切力, Piezo 1, 肾小球内皮细胞, 凋亡, Yoda 1

Abstract: Objective To investigate the effect of fluid shear(FS) on apoptosis of glomerular epithelial cells (GECs) and the role of Piezo 1 protein in it. Methods GECs (glomerular epithelial cells) of SD rat were cultured. Fluid shear stimulation was simulated by a Flexcell-T5000 tensiometer. Apoptosis level was detected by flow cytometry. The expression of Piezo 1 proteins in GECs was detected by immunofluorescence staining. The activating of Piezo 1 channels by fluid shear was observed using Ca²⁺ indicator (Cal-590 AM). The effect of Piezo 1 on apoptosis in GECs was analyzed after modulating the function or expression of Piezo 1 protein using the chemical activator Yoda1, the inhibitor GsMtx 4 was regulated by lentivirus Lv-shPiezo 1. Results Compared with the blank controlgroup, apoptosis increased in the fluid shear group (P＜0.05). The rate of apoptosis increased with the enhancing of fluid shear strength; Piezo 1 was commonly expressed in GECs. Fluid shear activated Piezo 1 channel and enhanced expression of Piezo 1. The agonist Yoda1 promoted the apoptosis of GECs GsMtx 4 inhibited the apoptosis induced by fluid shear. Lv-shPiezo 1 knocked down the expression of Piezo 1 in GECs and the apoptosis rate of GECs in the knockdown group was reduced as compared to that in the control group and Lv-Ctrl group(P＜0.05). Conclusions Fluid shear may promote apoptosis of GECs by activation of Piezo 1 and by enhancing expression of Piezo 1.

Key words: fluid shear, Piezo 1, glomerular epithelial cell, apoptosis, Yoda 1

中图分类号:

R692.5

罗梦琳, 郑峰, 季欣瑶, 祁彩虹, 肖雨珩, 牛长春. 流体剪切力通过激活Piezo 1蛋白促进肾小球内皮细胞凋亡[J]. 基础医学与临床, 2024, 44(9): 1236-1242.

LUO Menglin, ZHENG Feng, JI Xinyao, QI Caihong, XIAO Yuheng, NIU Changchun. Fluid shear promotes apoptosis of glomerular endothelial cells by activation of Piezo 1[J]. Basic & Clinical Medicine, 2024, 44(9): 1236-1242.

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流体剪切力通过激活Piezo 1蛋白促进肾小球内皮细胞凋亡

Fluid shear promotes apoptosis of glomerular endothelial cells by activation of Piezo 1

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