提高m6A去甲基化酶FTO表达抑制鼻咽癌细胞增殖

doi:10.16352/j.issn.1001-6325.2024.01.0057

基础医学与临床 ›› 2024, Vol. 44 ›› Issue (1): 57-62.doi: 10.16352/j.issn.1001-6325.2024.01.0057

提高m⁶A去甲基化酶FTO表达抑制鼻咽癌细胞增殖

廖镇城^1,2, 杨思懿¹, 吴平安^1*

1.香港大学深圳医院耳鼻咽喉头颈外科,广东深圳 518000;
2.深圳大学医学部,广东深圳 518000

收稿日期:2022-11-01 修回日期:2023-10-24 出版日期:2024-01-05 发布日期:2023-12-25
通讯作者: ^*:wupa@hku-szh.org
基金资助:
深圳市科技研发资金基础研究专项(JCYJ20220530142413031);香港大学深圳医院临床肿瘤科深圳市癌症转移与个体化治疗重点实验室 (ZDSYS20210623091811035)

Increased expression of m⁶A demethylase FTO inhibits the proliferation of nasopharyngeal carcinoma cells

LIAO Zhencheng^1,2, YANG Siyi¹, WU Ping'an^1*

1. Department of Surgery, Division of Otolaryngology, Head and Neck Surgery, the University of Hong Kong-Shenzhen Hospital, Shenzhen 518000;
2. Department of Medicine, Shenzhen University, Shenzhen 518000, China

Received:2022-11-01 Revised:2023-10-24 Online:2024-01-05 Published:2023-12-25
Contact: ^*:wupa@hku-szh.org

摘要/Abstract

摘要： 目的探究N6-甲基腺嘌呤(m⁶A)去甲基化酶人类脂肪和肥胖相关(FTO)蛋白在鼻咽癌(NPC)中的表达水平,以及过表达FTO对鼻咽癌体内外增殖的影响。方法用免疫组织化学检测鼻咽癌组织及癌旁组织中FTO蛋白的表达;筛选FTO的优势表达细胞株,构建过表达FTO细胞株,用软琼脂增殖实验验证;建立小鼠成瘤模型,测量肿瘤的生长速度。结果发现FTO在鼻咽癌组织中较癌旁组织低表达。FTO低表达促进了鼻咽癌细胞的增殖,而过表达FTO则可逆转这种作用。结论 FTO抑制鼻咽癌细胞的增殖过程,可为寻找鼻咽癌的治疗靶点提供实验依据,FTO 可能成为未来 NPC 的治疗靶点。

关键词: 鼻咽癌, N6-甲基腺嘌呤, 人类脂肪和肥胖相关蛋白, 细胞增殖

Abstract: Objective To investigate the expression of N6 methyladenine (m6A) demethylase human fat mass and obesity-associated(FTO) protein in nasopharyngeal carcinoma (NPC), and the effect of over-expression of FTO on the proliferation of nasopharyngeal carcinoma in vitro and in vivo. Methods Immunohistochemistry method was used to detect the expression of FTO protein in nasopharyngeal carcinoma tissues and para-cancerous tissues; The dominant expression cell line of FTO was screened, the over-expression FTO cell line was constructed. The cell proliferation was examined by soft-agar method. A mouse tumor model was developed for measurement of tumor growth. Results The expression of FTO in nasopharyngeal carcinoma tissues was lower than that in adjacent tissues. Low expression of FTO promoted proliferation of NPC cells, while over-expression of FTO reversed this effect. Conclusions FTO inhibits proliferation of nasopharyngeal carcinoma and this result may provide an experimental technology in searching therapeutic targets of chemotherapy for nasopharyngeal carcinoma.

Key words: nasopharyngeal carcinoma, N6-methyladenosine, human fat mass and obesity-associated proteins, cell proliferation

中图分类号:

R739.63

廖镇城, 杨思懿, 吴平安. 提高m⁶A去甲基化酶FTO表达抑制鼻咽癌细胞增殖[J]. 基础医学与临床, 2024, 44(1): 57-62.

LIAO Zhencheng, YANG Siyi, WU Ping'an. Increased expression of m⁶A demethylase FTO inhibits the proliferation of nasopharyngeal carcinoma cells[J]. Basic & Clinical Medicine, 2024, 44(1): 57-62.

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提高m⁶A去甲基化酶FTO表达抑制鼻咽癌细胞增殖

Increased expression of m⁶A demethylase FTO inhibits the proliferation of nasopharyngeal carcinoma cells

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