没食子酸减轻香烟烟雾诱导的小鼠气道炎性反应

doi:10.16352/j.issn.1001-6325.2023.11.1648

基础医学与临床 ›› 2023, Vol. 43 ›› Issue (11): 1648-1654.doi: 10.16352/j.issn.1001-6325.2023.11.1648

没食子酸减轻香烟烟雾诱导的小鼠气道炎性反应

赵芳^1*, 赵青², 刘玮琳³, 李菲⁴

河北省第八人民医院河北省老年病医院 1.呼吸肾内科;2.医教处,河北石家庄 050000;
3.河北医科大学第四医院感染性疾病科,河北石家庄 050000;
4.河北医科大学第一医院保健处, 河北石家庄 050000

收稿日期:2023-04-06 修回日期:2023-07-18 出版日期:2023-11-05 发布日期:2023-10-30
通讯作者: ^*tuteth438@21cn.com
基金资助:
2023年度河北省医学科学研究课题计划(20231194)

Gallic acid alleviates airway inflammation induced by cigarette smoke in mice

ZHAO Fang^1*, ZHAO Qing², LIU Weilin³, LI Fei⁴

1. Department of Respiratory and Renal Medicine;2. Department of Medical Education, Hebei Eighth People's Hospital/Hebei Geriatrics Hospital, Shijiazhuang 050000;
3. Department of Infectious Diseases, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050000;
4. Department of Health Care, the First Hospital of Hebei Medical University, Shijiazhuang 050000, China

Received:2023-04-06 Revised:2023-07-18 Online:2023-11-05 Published:2023-10-30
Contact: ^*tuteth438@21cn.com

摘要/Abstract

摘要： 目的研究没食子酸(GA)对香烟烟雾(CS)诱导的小鼠慢性阻塞性肺病(COPD)相关的肺部炎性反应的作用。方法构建CS诱导的小鼠模型。分析支气管肺泡灌洗液中的炎性细胞的数量和促炎细胞因子的水平。相应试剂盒检测肺组织匀浆中活性氧(ROS)、谷胱甘肽(GSH)、丙二醛(MDA)和蛋白羰基的活性。Western blot分析IL-13/STAT6通路活性。结果 GA抑制SC诱导的炎性细胞浸润(中性粒细胞、淋巴细胞和巨噬细胞)和促炎细胞因子(IL-6、TNF-α和IL-1β)的水平。GA可以减轻暴露于CS的小鼠肺组织氧化还原失衡。结论 GA有效地减轻COPD模型小鼠肺部炎性反应和氧化应激。

关键词: 没食子酸, IL-13/STAT6信号通路, 慢性阻塞性肺病, 气道炎性反应

Abstract: Objective To investigate the effect of gllic acid (GA) on the pulmonary inflammatory response associated with chronic obstructive pulmonary disease (COPD) induced by cigarette smoking (CS) in mice. Methods The mouse model induced by CS was constructed. The counting number of inflammatory cells and the level of proinflammatory cytokines in bronchoalveolar lavage fluid were analyzed. The ROS, GSH, MDA and protein carbonyl activity in lung tissue homogenate were determined by commercially available kits. Western blot was used to analyze IL-13/STAT6 pathway. Results GA inhibited SC-induced inflammatory cell infiltration (neutrophils, lymphocytes, and macrophages) and decreased the level of pro-inflammatory cytokines (IL-6, TNF-α, and IL-1β). GA alleviated the redox imbalance and CS-induced airway inflammation in lung tissue of CS exposed mice. The later might be mediated by by inhibiting IL-13/STAT6 signaling pathway. Conclusions GA effectively modulates pulmonary inflammation and oxidative stress in mice associated with COPD pathogenesis.

Key words: gallic acid, IL-13/STAT6 signaling pathway, chronic obstructive pulmonary disease, airway inflammation

中图分类号:

R563

赵芳, 赵青, 刘玮琳, 李菲. 没食子酸减轻香烟烟雾诱导的小鼠气道炎性反应[J]. 基础医学与临床, 2023, 43(11): 1648-1654.

ZHAO Fang, ZHAO Qing, LIU Weilin, LI Fei. Gallic acid alleviates airway inflammation induced by cigarette smoke in mice[J]. Basic & Clinical Medicine, 2023, 43(11): 1648-1654.

参考文献 15

[1]	Jia J, Conlon TM, Ballester Lopez C, et al. Cigarette smoke causes acute airway disease and exacerbates chronic obstructive lung disease in neonatal mice[J]. Am J Physiol Lung Cell Mol Physiol,2016,311:602-610.
[2]	Montalbano AM, Riccobono L, Siena L, et al. Cigarette smoke affects IL-17A, IL-17F and IL-17 receptor expression in the lung tissue: ex vivo and in vitro studies[J]. Cytokine,2015,76:391-402.
[3]	Nouri A, Salehi-Vanani N, Heidarian E. Antioxidant, anti-inflammatory and protective potential of gallic acid against paraquat-induced liver toxicity in male rats[J]. Avicenna J Phytomed,2021,11:633-644.
[4]	Ashrafizadeh M, Zarrabi A, Mirzaei S, et al. Gallic acid for cancer therapy: molecular mechanisms and boosting efficacy by nanoscopical delivery[J]. Food Chem Toxicol,2021,157:112576.doi: 10.1016/j.fct.2021.112576.
[5]	Wang X, Zhao H, Ma C, et al. Gallic acid attenuates aller-gic airway inflammation via suppressed interleukin-33 and group 2 innate lymphoid cells in ovalbumin-induced asthma in mice[J]. Int Forum Allergy Rhinol,2018,8:1284-1290.
[6]	Rahman I. Antioxidant therapies in COPD. Int J Chron Obstruct Pulmon Dis, 2006,1:15-29.
[7]	Barnes PJ. Oxidative stress-based therapeutics in COPD. Redox Biol, 2020,33:101544.doi: 10.1016/j.redox.2020.101544.
[8]	Nikbakht J, Hemmati AA, Arzi A, et al. Protective effect of gallic acid against bleomycin-induced pulmonary fibrosis in rats[J]. Pharmacol Rep,2015,67:1061-1067.
[9]	Karimi-Khouzani O, Heidarian E, Amini SA. Anti-inflammatory and ameliorative effects of gallic acid on fluoxetine-induced oxidative stress and liver damage in rats[J]. Pharmacol Rep,2017,69:830-835.
[10]	Panza VS, Wazlawik E, Ricardo Schutz G, et al. Consumption of green tea favorably affects oxidative stress markers in weight-trained men[J]. Nutrition, 2008,24:433-442.
[11]	Chiu HF, Lin TY, Shen YC, et al. Improvement of green tea polyphenol with milk on skin with respect to antioxidation in healthy adults: a double-blind placebo-controlled randomized crossover clinical trial[J]. Food Funct, 2016,7:893-901.
[12]	P LI, Bukowska B. Tea and coffee polyphenols and their biological properties based on the latest in vitro investigations[J]. Ind Crops Prod, 2022,175:114265. doi: 10.1016/j.indcrop.2021.114265.
[13]	Ahmad A, Shameem M, Husain Q. Altered oxidant-antioxidant levels in the disease prognosis of chronic obstructive pulmonary disease[J]. Int J Tuberc Lung Dis, 2013,17:1104-1109.
[14]	Chen CY, Chen KC, Yang TY, et al. Gallic acid induces a reactive oxygen species-provoked c-Jun NH2-terminal kinase-dependent apoptosis in lung fibroblasts[J]. Evid Based Complement Alternat Med, 2013,2013:613950.doi: 10.1155/2013/613950.
[15]	Yang Y, Li Q, Wei S, et al. STAT6/VDR Axis mitigates lung inflammatory injury by promoting Nrf2 signaling pathway[J]. Oxid Med Cell Longev, 2022,2022:2485250. doi: 10.1155/2022/2485250. .

没食子酸减轻香烟烟雾诱导的小鼠气道炎性反应

Gallic acid alleviates airway inflammation induced by cigarette smoke in mice

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