大鼠动脉螺旋血流的荧光成像

doi:10.16352/j.issn.1001-6325.2023.04.0583

基础医学与临床 ›› 2023, Vol. 43 ›› Issue (4): 583-587.doi: 10.16352/j.issn.1001-6325.2023.04.0583

大鼠动脉螺旋血流的荧光成像

戚曦¹, 李贝², 李宏义², 李岩松³, 赵立豪³, 郝优⁴, 于雪^{1, 5*}

1.北京大学第五临床医学院,北京 100730;
2.北京医院组织液循环研究中心国家老年医学中心中国医学科学院老年医学研究院,北京 100730;
3.清华大学航天航空学院工程力学系,北京 100084;
4.中国科学院计算技术研究所智能信息处理重点实验室,北京 100190;
5.北京医院心血管内科国家老年医学中心中国医学科学院老年医学研究院,北京 100730

收稿日期:2022-11-09 修回日期:2022-12-21 出版日期:2023-04-05 发布日期:2023-04-03
通讯作者: ^*yuxuemd@aliyun.com
基金资助:
中央高水平医院临床研究基金(BJ-2022-124,BJ-2022-113);中国医学科学院医学与健康科技创新工程(2021-I2M-1-050);国家自然科学基金(8205004)

Fluorescence imaging of arterial blood spiral laminar flow of rats

QI Xi¹, LI Bei², LI Hongyi², LI Yansong³, ZHAO Lihao³, HAO You⁴, YU Xue^1,5*

1. Peking University Fifth School of Clinical Medicine, Beijing 100730;
2. Center for Interstitial Fluid Circulation, Beijing Hospital, National Center of Gerontology;Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing 100730;
3. Department of Engineering Mechanics, School of Aerospace Engineering, Tsinghua University, Beijing 100084;
4. Key Laboratory of Intelligent Information Processing, Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100190;
5. Department of Cardiology, Beijing Hospital, National Center of Gerontology;Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing 100730, China

Received:2022-11-09 Revised:2022-12-21 Online:2023-04-05 Published:2023-04-03
Contact: ^*yuxuemd@aliyun.com

摘要/Abstract

摘要： 目的建立大鼠动脉螺旋血流的实时荧光成像法。方法随机将SD大鼠分为静脉造影组(股静脉注射,1 mL/kg)与小剂量动脉造影组(腹主动脉注射,0.1 mL/kg),其中小剂量动脉造影组又分为荧光素钠组、罗丹明B组、FITC-dextran组和量子点组(每组n=7)。通过体式荧光显微镜实时观察大鼠股动脉血液流动现象,用高速摄像机拍摄动态图像,通过图像阈值分割算法及形态学后处理算法标定荧光区域,测算大鼠股动脉螺旋血流的轴向速度与切向速度,并计算其螺距和壁剪切应力。结果与静脉造影组相比,小剂量动脉造影组可以清晰显示大鼠股动脉的血流呈顺时针螺旋样流动,在FITC-dextran造影剂组可计算出大鼠螺旋血流的轴向速度为(198.4±112.7)mm/s,切向速度为(3.3±0.8)mm/s,螺距为(126.1±76.3)mm,壁剪切应力为(9.5±5.6)Pa。每只大鼠重复3次注射,测得的轴向、切向速度结果无差异。结论实时荧光成像法可以用于在体观察大鼠动脉螺旋血流并对螺旋血流的血流动力学特征进行定量分析。

关键词: 动脉, 螺旋血流, 荧光成像, 壁剪切应力

Abstract: Objective To estatblish a real-time fluorescence imaging method for observing arterial blood spiral laminar flow. Methods Sprague Dawley rats were randomly divided into vein angiography group (femoral vein injection, 1 mL/kg) and low-dose arteriography group (abdominal aortic injection, 0.1 mL/kg). The low-dose arteriography group was further divided into fluorescein sodium group, rhodamine B group, FITC-dextran group and quantum dot group (n=7 for each group). The dynamic images were captured with high-speed camera. The fluorescence region was calibrated by image threshold segmentation algorithm and morphological post-processing algorithm. The axial velocity and tangential velocity of helical blood flow in rat femoral artery were measured, and the screw pitch and shear force were calculated. Results Compared with the vein angiography group, the low-dose arteriography group clearly showed the clockwise spiral laminar flow in the femoral artery of rats. In the FITC-dextran group, the axial velocity of blood spiral laminar flow was (198.4±112.7)mm/s, and the tangential velocity was(3.3±0.8)mm/s.The screw pitch was (126.1±76.3)mm, and the shear force was (9.5±5.6)Pa. The injections were repeated three times for each rat, and there was no significant difference among each injection. Conclusions Real-time fluorescence imaging can be used to observe arterial blood spiral laminar flow in vivo and to quantitatively analyze the hemodynamic characteristics of spiral laminar flow.

Key words: artery, spiral laminar flow, fluorescence imaging, wall shear stress

中图分类号:

R331

戚曦, 李贝, 李宏义, 李岩松, 赵立豪, 郝优, 于雪. 大鼠动脉螺旋血流的荧光成像[J]. 基础医学与临床, 2023, 43(4): 583-587.

QI Xi, LI Bei, LI Hongyi, LI Yansong, ZHAO Lihao, HAO You, YU Xue. Fluorescence imaging of arterial blood spiral laminar flow of rats[J]. Basic & Clinical Medicine, 2023, 43(4): 583-587.

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大鼠动脉螺旋血流的荧光成像

Fluorescence imaging of arterial blood spiral laminar flow of rats

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