关键词: 卵巢癌, lncRNA, 锌指E盒结合同源盒蛋白2-AS1(ZEB2-AS1)

Abstract: Objective To screen the valuable core(long non-coding RNAs,lncRNAs) from the tissue of ovarian cancer (OC) through expression profiling chip, and carry out expression verification and analysis. Methods Thirty-six tissue samples of ovarian cancer and of 22 normal ovaries were collected from January 2018 to June 2019 in the General Hospital of Ningxia Medical University and then expression profile chips were used to detect differentially expressed lncRNAs and mRNAs molecules, while real-time PCR was to verify the expression of lncRNAs. The expression of key core lncRNAs were measured by in situ hybridization, and the potential functions of lncRNAs were evaluated by bioinformatics. Results Compared with normal tissues, 4 982 different lncRNAs fragments were found in cancer tissues. The high expression of zinc finger E-box binding homeobox protein 2-AS1 (ZEB2-AS1) was the most significant one (P＜0.05); ZEB2-AS1 was over-expressed in multiple cancer tissues including liver cancer, colon cancer and kidney cancer;The expression level was correlated with tumor size, pathological grade, pathological type and clinical stage (P＜0.05).The high expression led to a decrease in overall survival and disease-free survival rate(P＜0.05). ZEB2-AS1 had microRNA binding sites, which was in agreement with the expression of the corresponding neighboring gene ZEB2. Conclusions The abnormal expression of ZEB2-AS1 in OC suggests potential research value in future.

Key words: ovarian cancer, lncRNA, zinc finger E-box binding homeobox protein 2-AS1(ZEB2-AS1)