LncRNA SNHG14调控miR-142-5p对寡聚态Aβ诱导的鼠源神经细胞系PC12损伤的影响

doi:10.16352/j.issn.1001-6325.2022.06.022

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (6): 913-919.doi: 10.16352/j.issn.1001-6325.2022.06.022

LncRNA SNHG14调控miR-142-5p对寡聚态Aβ诱导的鼠源神经细胞系PC12损伤的影响

沈杰¹, 严洁², 钟明¹, 庞睿娟¹, 罗永杰^3*

成都市第二人民医院 1.神经内科; 2.检验科, 四川成都 610017;
3.四川省医学科学院四川省人民医院神经内科, 四川成都 610072

收稿日期:2021-10-28 修回日期:2022-01-28 出版日期:2022-06-05 发布日期:2022-06-02
通讯作者: * m529mo@163.com
基金资助:
四川省科技厅科研基金重点项目(2019YFS0214);四川省卫计委科研基金重点项目(18ZD011)

Effect of lncRNA SNHG14 on oligomeric Aβ-induced injury in rat neural cell line PC12 through regulating miR-142-5p

SHEN Jie¹, YAN Jie², ZHONG Ming¹, PANG Rui-juan¹, LUO Yong-jie^3*

1. Department of Neurology; 2. Department of Laboratory, Chengdu Second People's Hospital, Chengdu 610017;
3. Department of Neurology, Sichuan Provincial People's Hospital, Sichuan Academy of Medical Sciences, Chengdu 610072,China

Received:2021-10-28 Revised:2022-01-28 Online:2022-06-05 Published:2022-06-02
Contact: * m529mo@163.com

摘要/Abstract

摘要： 目的探讨lncRNA SNHG14靶向miR-142-5p对寡聚态Aβ诱导的鼠源神经细胞系PC12损伤的影响。方法将PC12细胞分为对照组、模型组、模型+si-NC组、模型+si-SNHG14组、模型+miR-NC组、模型+miR-142-5p组、模型+si-SNHG14+anti-miR-NC组、模型+si-SNHG14+anti-miR-142-5p组。RT-qPCR检测SNHG14和miR-142-5p水平。CCK-8和流式细胞术测量细胞增殖抑制率和凋亡率。ELISA检测培养液中TNF-α、IL-6和IL-10水平。双荧素酶报告实验确定SNHG14和miR-142-5p的靶向关系。结果 AD患者血浆中SNHG14呈高表达(P＜0.05),miR-142-5p呈低表达(P＜0.05)。与对照组比较,模型组细胞SNHG14水平、增殖抑制率、凋亡率显著升高(P＜0.05),miR-142-5p水平显著降低(P＜0.05),培养液中TNF-α和IL-6水平显著升高(P＜0.05),IL-10水平显著降低(P＜0.05)。与model+si-NC组比较,model+si-SNHG14组细胞增殖抑制率、凋亡率显著降低(P＜0.05),培养液中TNF-α和IL-6水平显著降低(P＜0.05),IL-10水平显著升高(P＜0.05)。与model+miR-NC组比较,model+miR-142-5p组细胞增殖抑制率、凋亡率显著降低(P＜0.05),培养液中TNF-α和IL-6水平显著降低(P＜0.05),IL-10水平显著升高(P＜0.05)。miR-142-5p是SNHG14的直接靶点。与model+si-SNHG14+ anti-miR-NC组比较,model+ si-SNHG14+anti-miR-142-5p组细胞增殖抑制率、凋亡率显著升高(P＜0.05),培养液中TNF-α和IL-6水平显著升高(P＜0.05),IL-10水平显著降低(P＜0.05)。结论沉默SNHG14通过靶向促进miR-142-5p表达能够减轻寡聚态Aβ诱导的神经细胞损伤。

关键词: 阿尔茨海默病, SNHG14, miR-142-5p, 寡聚态Aβ, 神经细胞

Abstract: Objective To investigate the effect of lncRNA SNHG14 targeting at miR-142-5p on the neuronal damage induced by oligomeric Aβ. Methods PC12 cells were divided into control group, model group, model+si-NC group, model+si-SNHG14 group, model+miR-NC group, model+miR-142-5p group, model+si-SNHG14+anti-miR-NC group, and model+ si-SNHG14+anti-miR-142-5p group. The level of SNHG14 and miR-142-5p was calculated by RT-qPCR. CCK-8 method and flow cytometry were applied to detect inhibition rate on cell proliferation and apoptosis rate. ELISA method was used to detect TNF-αand IL-6 in the culture. Dual luciferase report assay was used to find the targeting relationship between SNHG14 and miR-142-5p. Results SNHG14 was highly expressed (P＜0.05)while miR-142-5p was lowly expressed (P＜0.05) in plasma of AD patients. Compared with the control group, the SNHG14 level, cell proliferation inhibition rate, and apoptosis rate in the model group were significantly increased (P＜0.05), the level of miR-142-5p was significantly reduced (P＜0.05), and the levels of TNF-α and IL-6 in the culture medium were significantly increased (P＜0.05), IL-10 level was significantly decreased (P＜0.05). Compared with the model+si-NC group, the cell proliferation inhibition rate and apoptosis rate in the model+si-SNHG14 group were significantly reduced (P＜0.05), the level of TNF-α and IL-6 in the culture medium was significantly reduced (P＜0.05), IL-10 level was significantly increased(P＜0.05). Compared with the model+miR-NC group, the cell proliferation inhibition rate and apoptosis rate in the model+miR-142-5p group were significantly reduced (P＜0.05), and the levels of TNF-α and IL-6 in the culture medium were significantly reduced (P＜0.05) and IL-10 level was significantly increased (P＜0.05). miR-142-5p was a direct target of SNHG14. Compared with the model+si-SNHG14+ anti-miR-NC group, the cell proliferation inhibition rate and apoptosis rate in the model+ si-SNHG14+anti-miR-142-5p group were significantly increased(P＜0.05), and the levels of TNF-α and IL-6 in the culture medium were significantly increased (P＜0.05), IL-10 level was significantly decreased (P＜0.05). Conclusions Silencing SNHG14 may alleviate oligomeric Aβ-induced neuronal damage by targeting at and promoting miR-142-5p expression.

Key words: Alzheimer's disease, SNHG14, miR-142-5p, oligomeric Aβ, nerve cell

中图分类号:

R749.1+6

沈杰, 严洁, 钟明, 庞睿娟, 罗永杰. LncRNA SNHG14调控miR-142-5p对寡聚态Aβ诱导的鼠源神经细胞系PC12损伤的影响[J]. 基础医学与临床, 2022, 42(6): 913-919.

SHEN Jie, YAN Jie, ZHONG Ming, PANG Rui-juan, LUO Yong-jie. Effect of lncRNA SNHG14 on oligomeric Aβ-induced injury in rat neural cell line PC12 through regulating miR-142-5p[J]. Basic & Clinical Medicine, 2022, 42(6): 913-919.

参考文献

[1] Lane CA, Hardy J, Schott JM. Alzheimer's disease[J]. Eur J Neurol, 2018, 25: 59-70.
[2] Lee S, Youn K, Jun M. Major compounds of red ginseng oil attenuate Aβ25-35-induced neuronal apoptosis and inflammation by modulating MAPK/NF-κB pathway[J]. Food Funct, 2018, 9: 4122-4134.
[3] Dong LX, Zhang YY, Bao HL, et al. LncRNA NEAT1 promotes Alzheimer's disease by down regulating micro-27a-3p[J]. Am J Transl Res, 2021, 13: 8885-8896.
[4] He Y, Qiang Y. Mechanism of autonomic exercise improving cognitive function of Alzheimer's disease by regulating lncRNA SNHG14[J]. Am J Alzheimers Dis Other Demen, 2021, 36:7681-7691.
[5] Chen J, Jiang C, Du J, et al. MiR-142-5p protects against 6-OHDA-induced SH-SY5Y cell injury by downregulating BECN1 and autophagy[J]. Dose Response, 2020, 18: 7016-7026.
[6] 张佩玲. 原儿茶酸抑制Aβ诱导的PC12细胞的毒性作用及机制研究[D]. 广州中医药大学, 2016: 1-43.
[7] Zhuang J, Cai P, Chen Z, et al. Long noncoding RNA MALAT1 and its target microRNA-125b are potential biomarkers for Alzheimer's disease management via interac-tions with FOXQ1, PTGS2 and CDK5[J]. Am J Transl Res, 2020, 12: 5940-5954.
[8] Ke S, Yang Z, Yang F, et al. Long Noncoding RNA NEAT1 aggravates Aβ-induced neuronal damage by targeting miR-107 in Alzheimer's disease[J]. Yonsei Med J, 2019, 60: 640-650.
[9] Tang Y, Xiong R, Wu AG, et al. Polyphenols derived from lychee seed suppress Aβ (1-42)-induced neuroinflammation[J]. Int J Mol Sci, 2018, 19: 2109-2119.
[10] Zhong Y, Yu C, Qin W. LncRNA SNHG14 promotes inflammatory response induced by cerebral ischemia/reperfusion injury through regulating miR-136-5p /ROCK1[J]. Cancer Gene Ther, 2019, 26: 234-247.
[11] Zhou S, Zhang D, Guo J, et al. Knockdown of SNHG14 alleviates MPP+-induced injury in the cell model of Parkinson's disease by targeting the miR-214-3p/KLF4 axis[J]. Front Neurosci, 2020, 14: 930-940.
[12] 鲁帅奇, 杨凌博, 秦帅锋, 等. lncRNA SNHG14通过miR-217-5p/FHIT分子轴抑制膀胱癌细胞的增殖和侵袭能力[J]. 实用医学杂志, 2020, 36: 1903-1907.
[13] 陈艳霞, 哈尼克孜·吐尔逊, 梁凌云, 等. 长链非编码RNA SNHG14/miR-211对宫颈癌细胞的增殖、侵袭能力的影响[J]. 现代生物医学进展, 2021, 21: 2622-2625, 2632.
[14] Du J, Yang ST, Liu J, et al. Silence of lncRNA GAS5 protects cardiomyocytes H9c2 against hypoxic injury via sponging miR-142-5p[J]. Mol Cells, 2019, 42: 397-405.
[15] Tang B, Li W, Ji T, et al. Downregulation of XIST ameliorates acute kidney injury by sponging miR-142-5p and targeting PDCD4[J]. J Cell Physiol, 2020, 235: 8852-8863.

LncRNA SNHG14调控miR-142-5p对寡聚态Aβ诱导的鼠源神经细胞系PC12损伤的影响

Effect of lncRNA SNHG14 on oligomeric Aβ-induced injury in rat neural cell line PC12 through regulating miR-142-5p

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	秦晓莉, 赵琳娜, 付榕, 郭玉莹, 徐士欣. 星形胶质细胞吞噬作用在阿尔茨海默病中的研究进展[J]. 基础医学与临床, 2024, 44(8): 1180-1184.
[2]	高璐, 蔡孟华, 许依, 何维, 陈慧, 张建民. 过表达膜定位IL-3的293T细胞外泌体的纯化及体外功能验证[J]. 基础医学与临床, 2024, 44(7): 947-953.
[3]	陈小坤, 包新杰, 高俊, 李永宁. 神经干细胞移植治疗阿尔兹海默病的机制研究进展[J]. 基础医学与临床, 2023, 43(7): 1148-1151.
[4]	刘诗雨, 刘宇健. 组织激肽释放酶8在神经系统疾病中作用的研究进展[J]. 基础医学与临床, 2023, 43(4): 660-664.
[5]	王远卓, 郑清月, 张翰林, 仇文颖, 王涛, 马超. 小胶质细胞在阿尔茨海默病神经炎性反应中作用的研究进展[J]. 基础医学与临床, 2022, 42(8): 1310-1313.
[6]	徐娟, 李晔. 铁死亡在阿尔茨海默病中作用的研究进展[J]. 基础医学与临床, 2022, 42(7): 1129-1133.
[7]	路亚岚, 周澧, 韩云林, 王克维, 秦川. 冈田酸诱导人神经母细胞瘤细胞系SH-SY5Y损伤[J]. 基础医学与临床, 2022, 42(6): 927-932.
[8]	孙健茹, 仇文颖, 钱晓菁, 马超. Fc-gamma Ⅰ型受体在阿尔茨海默病患者和模型小鼠脑皮质的表达[J]. 基础医学与临床, 2022, 42(5): 740-744.
[9]	孙静瑶, 佟伟民, 牛亚梅. mRNA转录后调控异常在阿尔茨海默病发病机制中的研究进展[J]. 基础医学与临床, 2021, 41(5): 749-752.
[10]	孙添怡, 刘帆, 仇文颖, 马超. piRNAs在阿尔茨海默病患者脑中的差异表达[J]. 基础医学与临床, 2021, 41(4): 533-538.
[11]	王紫涵, 罗金定, 田英入, 奉水东, 凌宏艳. 小胶质细胞引起和促进阿尔茨海默病的研究进展[J]. 基础医学与临床, 2021, 41(2): 277-281.
[12]	刁华琼, 李小黎, 林亮吟, 刘海鹏, 陈弘婧, 丁海月, 魏丹. 外泌体microRNAs与阿尔茨海默病关系的研究进展[J]. 基础医学与临床, 2020, 40(11): 1542-1545.
[13]	曾利敏, 鲁召辉, 周丽平, 朱超霞. 甲基莲心碱通过调控miR-29a-3p/AQP4表达抑制Aβ1-42致PC12细胞的损伤[J]. 基础医学与临床, 2020, 40(1): 83-91.
[14]	杜亚云刘晓丽朱席琳伍晓盼刘英. 胱硫醚β合成酶通过ATG5巯基化促进自噬缓解阿尔茨海默病[J]. 基础医学与临床, 2019, 39(7): 983-988.
[15]	刘建玉夏春娥赵雪梅梁平. CB-Aβ抗体偶联物抑制痴呆小鼠脑内Aβ纤维斑块形成[J]. 基础医学与临床, 2013, 33(7): 834-839.