放射治疗后宫颈癌组织内差异表达基因鉴定

doi:10.16352/j.issn.1001-6325.2022.02.002

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (2): 215-220.doi: 10.16352/j.issn.1001-6325.2022.02.002

放射治疗后宫颈癌组织内差异表达基因鉴定

白静^1#*, 刘娜^2,3,4#, 丁力¹, 雷丽¹, 倪瑞¹, 闫少春^2*, 邵国^2,3,4*

1.包头市肿瘤医院放射治疗科,内蒙古包头 014030;
2.包头医学院内蒙古自治区低氧转化医学重点实验室,内蒙古包头 014060;
3.首都医科大学宣武医院低氧适应转化医学北京重点实验室,北京 100053;
4.包头医学院第二附属医院内蒙古自治区消化疾病研究所检验科,内蒙古包头 014030

收稿日期:2020-11-13 修回日期:2021-06-08 出版日期:2022-02-05 发布日期:2022-01-24
通讯作者: ^* crystal_bj@sohu.com; yanshaochunbtyxy@163.com; shao_guo_china@163.com
作者简介:^#对本文有相同贡献
基金资助:
国家自然科学基金(81660307,82060337);内蒙古自然科学基金(2018MS08052,2020MS08010);包头医学院基金(BYJJ-YF201717)

Identification of differentially expressed genes in cervical carcinoma after radiotherapy

BAI Jing^1#*, LIU Na^2,3,4#, DING Li¹, LEI Li¹, NI Rui¹, YAN Shao-chun^2*, SHAO Guo^2,3,4*

1. Department of Radiptherapy, Baotou Tumor Hospital,Baotou 014030;
2. Inner Mongolia Key laboratory of Hypoxic Translational Medicine,Baotou Medical College,Baotou 014060;
3. Beijing Key Laboratory of Hypoxic Conditioning Translational Medicine, Xuanwu Hospital,Capital Medical University,Beijing 100053;
4. Institute of Digestive Diseases of Inner Mongolia Autonomous Region, Department of Laboratory Medicine, the Second Affiliated Hospital of Baotou Medical College,Baotou 014030,China

Received:2020-11-13 Revised:2021-06-08 Online:2022-02-05 Published:2022-01-24
Contact: ^* crystal_bj@sohu.com; yanshaochunbtyxy@163.com; shao_guo_china@163.com

摘要/Abstract

摘要： 目的研究放射治疗对宫颈癌组织基因表达的影响,探讨差异表达基因对放射治疗敏感及在抗拒中的作用。方法患者接受相同的放疗模式:全盆腔外照射总剂量45戈瑞(Gy),共25次(45 Gy/25 f),腔内近距离内照射每次剂量5～6 Gy,共治疗5～6次。分别在近距离放射治疗前、近距离放射治疗中、近距离放射治疗结束时留取标本。高通量分析近距离照射前,照射中和照射后基因表达的情况;real-time PCR 及Western blot验证与放射敏感和耐受相关的基因。结果生物信息学显示DNA损伤基因表达增加(P＜0.05),real-time PCR 及Western blot分析DNA损伤相关基因GADD45表达较治疗前增加(P＜0.05)。结论放射治疗可引起众多宫颈癌组织基因表达变化,其中GADD45表达增加可能在放射治疗中起到重要作用。

关键词: 宫颈癌, 放射治疗, 生物信息学

Abstract: Objective To study the effect of radiotherapy on gene expression in cervical cancer tissues and to find the role of differentially expressed genes in sensitivity and resistance to radiotherapy. Methods The patients all received the same radiotherapy as follows: total pelvic external radiation 45 Gy/25 f,intracavitary brachytherapy 5-6 Gy/f with total dosage as 5-6 f.The specimens were collected before treatment and by the end of treatment.High-throughput analysis of gene expression was performed before, during and after irradiation; real-time PCR and Western blot was applied to identify the genes related to radiation sensitivity and tolerance. Results Bioinformatics analysis showed an increased expression of DNA damage genes.The expression of DNA damage related genes GADD45 was increased as found by real-time PCR and Western blot. Conclusions Radiotherapy changes the gene expression in cervical cancer tissues and the increase of GADD45 expression may play an important role in radiotherapy.

Key words: cervical cancer, radiotherapy, bioinformatics

中图分类号:

R737.33

白静, 刘娜, 丁力, 雷丽, 倪瑞, 闫少春, 邵国. 放射治疗后宫颈癌组织内差异表达基因鉴定[J]. 基础医学与临床, 2022, 42(2): 215-220.

BAI Jing, LIU Na, DING Li, LEI Li, NI Rui, YAN Shao-chun, SHAO Guo. Identification of differentially expressed genes in cervical carcinoma after radiotherapy[J]. Basic & Clinical Medicine, 2022, 42(2): 215-220.

参考文献

[1]Ueno S,Sudo T,Oka N,et al.Absence of human papillomavirus infection and activation of PI3K-AKT pathway in cervical clear cell carcinoma[J].Int J Gynecol Cancer,2013,23:1084-1091.
[2]Vu M,Yu J,Awolude OA,et al.Cervical cancer worldwide[J].Curr Probl Cancer,2018,42:457-465.
[3]Cohen PA,Jhingran A,Oaknin A,et al.Cervical cancer[J].Lancet,2019,393:169-182.
[4]Bogari NM.Next generation sequencing (NGS) in glucose-6-phosphate dehydrogenase (G6PD) deficiency studies[J].Bioinformation,2016,12:41-43.
[5]Cui J,Liu N,Chang Z,et al.Exosomal microrna-126 from ripc serum is involved in hypoxia tolerance in sh-sy5y cells by downregulating dnmt3b[J].Mol Ther Nucleic Acids,2020,20:649-660.
[6]Siegel RL,Miller KD,Jemal A.Cancer statistics,2020[J].CA Cancer J Clin,2020,70:7-30.
[7]Serkies K,Jassem J.Systemic therapy for cervical carcinoma - current status[J].Chin J Cancer Res,2018,30:209-221.
[8]Arita T,Asai J,Taura M,et al.Squamous cell carcinoma of neck metastasized to the lung and cervical lymph nodes,successfully treated with systemic docetaxel and local radiation therapy to the neck in a renal transplant patient[J].J Dermatol,2017,44:e346-e347.
[9]Ueno T,Monzen S,Chiba M,et al.Screening for biological marker of dose-optimization in cancer radiotherapy[J].Nihon Hoshasen Gijutsu Gakkai Zasshi,2018,74:459-464.
[10]Fan Y,Meng Y,Yang S,et al.Screening of cervical cancer with self-collected cervical samples and next-generation sequencing[J].Dis Markers,2018,2018:4826547.doi:10.1155/2018/4826547.
[11]Garcia V,Garcia JM,Pena C,et al.The GADD45,ZBRK1 and BRCA1 pathway:quantitative analysis of mRNA expression in colon carcinomas[J].J Pathol,2005,206:92-99.
[12]Li C,Ming Y,Wang Z,et al.GADD45α alleviates acetaminophen-induced hepatotoxicity by promoting AMPK activation[J].Cell Mol Life Sci,2019,76:129-145.
[13]Verzella D,Bennett J,Fischietti M,et al.GADD45β loss ablates innate immunosuppression in cancer[J].Cancer Res,2018,78:1275-1292.
[14]Brito DVC,Kupke J,Gulmez Karaca K,et al.Mimicking age-associated gadd45gamma dysregulation results in memory impairments in young adult mice[J].J Neurosci,2020,40:1197-1210.
[15]Santucci MA,Barbieri E,Frezza G,et al.Radiation-induced gadd45 expression correlates with clinical res-ponse to radiotherapy of cervical carcinoma[J].Int J Radiat Oncol Biol Phys,2000,46:411-416.

放射治疗后宫颈癌组织内差异表达基因鉴定

Identification of differentially expressed genes in cervical carcinoma after radiotherapy

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