血清miR-181b和miR-27a表达与颈动脉粥样硬化程度相关

基础医学与临床 ›› 2021, Vol. 41 ›› Issue (8): 1133-1138.

血清miR-181b和miR-27a表达与颈动脉粥样硬化程度相关

张传西^*, 张宇, 郭莎莎, 厉菁, 袁义强, 王明杰

郑州市第七人民医院心内科, 河南郑州 450000

收稿日期:2020-06-23 修回日期:2020-12-20 出版日期:2021-08-05 发布日期:2021-07-21
通讯作者: *hxjh_25@163.com
基金资助:
河南省医学科技攻关计划(2017T02096)

Correlation of serum miR-181b and miR-27a expression with the severity of carotid atherosclerosis

ZHANG Chuan-xi^*, ZHANG Yu, GUO Sha-sha, LI Jing, YUAN Yi-qiang, WANG Ming-jie

Department of Cardiology, Zhengzhou Seventh People's Hospital, Zhengzhou 450000, China

Received:2020-06-23 Revised:2020-12-20 Online:2021-08-05 Published:2021-07-21
Contact: *hxjh_25@163.com

摘要/Abstract

摘要： 目的分析血清微小RNA(miR)-181b、miR-27a表达与颈动脉粥样硬化(CAS)病变程度的关系。方法选取血脂异常患者102例,健康人100名为对照组,RT-qPCR检测血清miR-181b和miR-27a的表达。对比血脂异常患者与对照组血清miR-181b、miR-27a表达差异,分析血脂异常患者发生CAS的影响因素及血清miR-181b、miR-27a表达与CAS病变程度的相关性。结果血脂异常患者的血清miR-181b相对表达量低于对照组,而miR-27a相对表达量高于对照组(P＜0.05);硬化组血清miR-181b相对表达量低于未硬化组,miR-27a相对表达量、hs-CRP、LDL-C水平、高血压占比高于未硬化组(P＜0.05),Logistic回归分析显示以上指标均是血脂异常患者发生CAS的独立影响因素(P＜0.05);ROC显示血清miR-181b、miR-27a相对表达量预测血脂异常患者发生CAS的灵敏度分别为84.75%、81.36%,特异度分别为76.74%、74.42%,准确度分别为81.37%、78.43%,AUC分别为0.791、0.750;Pearson相关性分析显示血清miR-181b 相对表达量与Crouse积分呈负相关(P＜0.05),miR-27a相对表达量与Crouse积分呈正相关(P＜0.05)。结论血清miR-181b与miR-27a在血脂异常患者表达异常,且二者均是CAS发生的独立影响因素,其表达水平与病情严重程度有关。

关键词: 颈动脉粥样硬化, 血脂异常, 微小RNA-181b, 微小RNA-27a

Abstract: Objective To identify the relationship between expression of serum microRNA (miR)-181b, miR-27a and the severity of carotid atherosclerosis (CAS). Methods Totally 102 patients with dyslipidemia were selected and 100 healthy people as controls.RT-qPCR was used to detect the expression of serum miR-181b and miR-27a. The expression of miR-181b and miR-27a of patients with dyslipidemia was compared with that of the control group. The influencing factors of the occurrence of CAS in patients with dyslipidemia and the correlation between serum miR-181b, miR-27a levels and the degree of CAS lesions were investigated. Results The relative expression of miR-181b in dyslipidemia patients was lower than that of the control group, while the relative expression of miR-27a was higher than that of the control group (P＜0.05). The expression of miR-181b in the cirrhosis group was lower than that in the non-cirrhotic group, and the relative expression of miR-27a, hs-CRP, LDL-C levels, and the proportion of hypertension were higher than those in the non-cirrhotic group (P＜0.05). Logistic regression analysis showed that the above indicators were independent factors influencing the occurrence of CAS in patients with dyslipidemia(P＜0.05). ROC showed that the sensitivity of relative expression of miR-181b and miR-27a in serum was 84.75% and 81.36% in the predicting the occurrence of CAS in patients with dyslipidemia, the specificity was 76.74% and 74.42%, and the accuracy was 81.37% and 78.43%, respectively, AUC was 0.791, 0.750 respectively. Pearson correlation analysis showed that the relative expression of serum miR-181b was negatively correlated with Crouse score(P＜0.05), and the relative expression of miR-27a was positively correlated with Crouse score(P＜ 0.05). Conclusions The expression of miR-181b and miR-27a in serum is abnormal in patients with dyslipidemia, and both of them are independent influencing factors of CAS, and their expression level is positively related to the severity of the disease.

Key words: carotid atherosclerosis, dyslipidemia, miR-181b, miR-27a

中图分类号:

R543.4

张传西, 张宇, 郭莎莎, 厉菁, 袁义强, 王明杰. 血清miR-181b和miR-27a表达与颈动脉粥样硬化程度相关[J]. 基础医学与临床, 2021, 41(8): 1133-1138.

ZHANG Chuan-xi, ZHANG Yu, GUO Sha-sha, LI Jing, YUAN Yi-qiang, WANG Ming-jie. Correlation of serum miR-181b and miR-27a expression with the severity of carotid atherosclerosis[J]. Basic & Clinical Medicine, 2021, 41(8): 1133-1138.

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