Cajal间质细胞数量在反复膀胱炎大鼠膀胱中增加

基础医学与临床 ›› 2020, Vol. 40 ›› Issue (9): 1237-1241.

Cajal间质细胞数量在反复膀胱炎大鼠膀胱中增加

高峰¹, 张士更², 张楠², 文甲明², 虞旗旗¹, 黄亚胜^1*

1.杭州市中医院泌尿外科, 浙江杭州 310007;
2.浙江大学医学院附属第二医院泌尿外科, 浙江杭州 310009

收稿日期:2019-08-20 修回日期:2020-01-03 出版日期:2020-09-05 发布日期:2020-09-04
通讯作者: ^*KK2158@163.com
基金资助:
杭州市科技发展计划项目(20191203B138)

Number increase of Cajal interstitial cells in bladder of rats with recurrent cystitis

GAO Feng¹, ZHANG Shi-geng², ZHANG Nan², WEN Jia-ming², YU Qi-qi¹, HUANG Ya-sheng^1*

1. Department of Urology,Hangzhou Hospital of Traditional Chinese Medicine, Hangzhou 310007;
2. Department of Urology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China

Received:2019-08-20 Revised:2020-01-03 Online:2020-09-05 Published:2020-09-04
Contact: ^*KK2158@163.com

摘要/Abstract

摘要： 目的研究Cajal间质细胞(ICCs)在大鼠反复膀胱炎相关膀胱过度活动症(OAB)膀胱中的数量变化。方法将大鼠分为对照组和实验组[大肠埃希菌(E.coli)1×10⁸～1.2×10⁸ CFU/mL,100 μL膀胱灌注],通过尿检和尿流动力学测定及HE染色膀胱组织明确OAB建模成功;用免疫荧光法观察膀胱组织c-kit阳性的ICCs,Western blot检测干细胞因子(SCF)及c-kit蛋白表达水平。结果实验组大鼠较对照组ICCs网格状分布紊乱,数量明显增加(P＜0.05),干细胞因子(SCF)及c-kit蛋白表达明显升高(P＜0.05)。结论 ICCs在反复膀胱炎膀胱中明显升高。

关键词: 膀胱过度活动症, 卡哈尔(Cajal)间质细胞, 干细胞因子, c-kit受体酪氨酸激酶

Abstract: Objective To explore the changes of interstitial cells of Cajal(ICCs) in the over active bladder(OAB) rats caused by recurrent cystitis. Methods Rats were divided into control group and experimental group(E.coli 1×10⁸~1.2×10⁸ CFU/mL, 100 μL bladder perfusion), The OAB model was confirmed by urine test, urine flow dynamics and HE staining of bladder tissue. The immunofluorescence staining method was performed to detect c-kit positive ICCs in bladder tissue. Western blot was used to determine the expression of SCF/c-kit protein. Results Compared with the control group, the quantity of ICCs in the experimental group increased significantly (P＜0.05), and the protein expression of SCF and c-kit increased significantly (P＜0.05). Conclusions ICCs increase significantly in the bladder with recurrent cystitis.

Key words: over active bladder, interstitial cells of Cajal, stem cell factor(SCF), c-kit receptor tyrosine kinase

中图分类号:

Q491.1

高峰, 张士更, 张楠, 文甲明, 虞旗旗, 黄亚胜. Cajal间质细胞数量在反复膀胱炎大鼠膀胱中增加[J]. 基础医学与临床, 2020, 40(9): 1237-1241.

GAO Feng, ZHANG Shi-geng, ZHANG Nan, WEN Jia-ming, YU Qi-qi, HUANG Ya-sheng. Number increase of Cajal interstitial cells in bladder of rats with recurrent cystitis[J]. Basic & Clinical Medicine, 2020, 40(9): 1237-1241.

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