IL-17通过NF-κB信号通路参与胃癌发生发展的免疫调控

基础医学与临床 ›› 2020, Vol. 40 ›› Issue (7): 923-928.

IL-17通过NF-κB信号通路参与胃癌发生发展的免疫调控

李春波^1,2, 苏韫^1,2*, 龚红霞^1,2, 薛轩^1,2, 李婷婷^1,2, 牛世伟^1,2, 聂蓬³

1.甘肃中医药大学基础医学院,甘肃兰州 730000;
2.甘肃中医药大学甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室,甘肃兰州 730000;
3.甘肃省武威肿瘤医院武威医学科学院胃外科, 甘肃武威 733000

收稿日期:2020-01-19 修回日期:2020-04-27 出版日期:2020-07-05 发布日期:2020-06-29
通讯作者: ^*suyungszy@126.com
基金资助:
国家自然科学基金(81660744)

Immune regulation of IL-17 through NF-κB signaling pathway involved in the development of gastric cancer

LI Chun-bo^1,2, SU Yun^1,2*, GONG Hong-xia^1,2, XUE Xuan^1,2, LI Ting-ting^1,2, NIU Shi-wei^1,2, NIE Peng³

1. School of Basic Medicine, Gansu University of Traditional Chinese Medicine,Lanzhou 730000;
2. Provincial-Level Key Laboratory for Molecular Medicine of Major Diseases and Study on Prevention and Treatment of Traditional Chinese Medicine, Gansu University of Chinese Medicine, Lanzhou 730000;
3. Department of Gastric Surgery, Wuwei Academy of Medical Sciences, Gansu Province Wuwei Cancer Hospital,Wuwei 733000,China

Received:2020-01-19 Revised:2020-04-27 Online:2020-07-05 Published:2020-06-29
Contact: ^*suyungszy@126.com

摘要/Abstract

摘要： 目的探讨白介素-17(IL-17)通过NF-κB信号通路参与胃癌发生的分子机制。方法选取甘肃省武威肿瘤医院胃癌患者30例及同一时间在医院体检的健康人30名;酶联免疫吸附法检测胃癌患者及健康人血清中IL-17、IL-6和IL-8的表达;用HE染色法观察胃癌患者胃组织病理学变化;免疫组织化学法检测胃癌患者的胃癌组织、癌旁组织及远离癌组织的胃组织中IL-17、NF-κB p65、IKK-β和IL-6的表达。Western blot检测胃癌患者的胃癌组织、癌旁组织及远离癌组织的胃组织IL-17、NF-κBp65、pNF-κB p65、IKK-β和IL-6的表达。结果与对照组相比,IL-17、IL-6和IL-8与在胃癌患者血清中表达上调(P＜0.05);IL-17、NF-κB p65、IKK-β和IL-6在胃癌组织中的表达明显高于正常及癌旁组织(P＜0.05)。与正常胃组织相比,胃癌组织中IL-17、NF-κB p65、pNF-κB p65、IKK-β和IL-6表达增加(P＜0.05);癌旁组织中pNF-κB p65、IKK-β和IL-6表达增加(P＜0.05)。与癌旁组织相比,胃癌组织中IL-17、NF-κB p65、pNF-κB p65、IKK-β和IL-6表达增加(P＜0.05)。结论 IL-17可能通过NF-κB信号通路刺激IL-6参与胃癌的免疫调控以及促进胃癌的发生发展,为胃癌发病机制提供新的见解。IL-17可能作为胃癌的临床治疗潜在靶点。

关键词: 胃癌, IL-17, NF-κB信号通路

Abstract: Objective To investigate the molecular mechanism of interleukin-17 in the development of gastric cancer through NF-κB signaling pathway. Methods Thirty patients with gastric cancer in Wuwei Cancer Hospital of Gansu province and 30 control individuals who had physical examination in the hospital at the same time were selected. The expression of IL-17,IL-6 and IL-8 in serum of patients with gastric cancer and control was detected by ELISA.The pathological changes of gastric cancer were observed by microscopy with HE staining; Immunohisto-chemistry method was used to detect the expression of IL-17, NF-κB p65, IKK-β and IL-6 in gastric cancer tissues, adjacent tissues and gastric tissues far from cancer tissues.The expressions of IL-17, NF-κB p65, pNF-κB p65, IKK-β and IL-6 in gastric cancer tissues, adjacent tissues and gastric tissues far from cancer tissues were detected by Western blot. Results IL-17, IL-6 and IL-8 were up-regulated in serum of gastric cancer patients compared with healthy controls group(P＜0.05). The expressions of IL-17, NF-κB p65, IKK-β and IL-6 in gastric cancer tissues were significantly higher than that in normal and adjacent groups (P＜0.05). The expressions of IL-17, NF-κB p65, pNF-κB p65, IKK-β and IL-6 were increased in gastric cancer tissues compared with normal gastric tissue (P＜0.05); the expressions of pNF-κB p65, IKK-β and IL-6 increased in peri-cancer tissues compared with normal gastric tissue (P＜0.05).The expressions of IL-17, NF-κB p65, pNF-κB p65, IKK-β and IL-6 were increased in gastric cancer tissues compared with adjacent tissues(P＜0.05). Conclusions IL-17 can stimulate IL-6 to function in regulation of gastric cancer and promote the development of gastric cancer through the NF-κB p65 signaling pathway.This result may provide new insights into the pathogenesis of gastric cancer and suggests that IL-17 may be a potential target of gastric cancer treatment.

Key words: gastric cancer, IL-17, NF-κB signaling pathway

中图分类号:

R735.2

李春波, 苏韫, 龚红霞, 薛轩, 李婷婷, 牛世伟, 聂蓬. IL-17通过NF-κB信号通路参与胃癌发生发展的免疫调控[J]. 基础医学与临床, 2020, 40(7): 923-928.

LI Chun-bo, SU Yun, GONG Hong-xia, XUE Xuan, LI Ting-ting, NIU Shi-wei, NIE Peng. Immune regulation of IL-17 through NF-κB signaling pathway involved in the development of gastric cancer[J]. Basic & Clinical Medicine, 2020, 40(7): 923-928.

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