肠易激综合征患儿肠道菌群可促进小鼠肠道酸敏感离子通道蛋白的表达

基础医学与临床 ›› 2020, Vol. 40 ›› Issue (2): 173-177.

肠易激综合征患儿肠道菌群可促进小鼠肠道酸敏感离子通道蛋白的表达

赵军梅, 袁丽萍^*, 蔡洁, 李文亚, 朱克然, 桂明

安徽医科大学第一附属医院儿科, 安徽合肥 230000

收稿日期:2019-03-05 修回日期:2019-09-02 出版日期:2020-02-05 发布日期:2020-02-05
通讯作者: ^*yuanliping3986@163.com
基金资助:
国家自然科学基金(81471617)

Intestinal flora of children with irritable bowel syndrome can promote the expression of intestinal acid-sensitive ion channel proteins in mice

ZHAO Jun-mei, YUAN Li-ping^*, CAI Jie, LI Wen-ya, ZHU Ke-ran, Gui Ming

Department of Pediatrics, the First Affiliated Hospital of Anhui Medical University,Hefei 230000, China

Received:2019-03-05 Revised:2019-09-02 Online:2020-02-05 Published:2020-02-05
Contact: ^*yuanliping3986@163.com

摘要/Abstract

摘要： 目的探讨肠易激综合征(IBS)患儿肠道菌群对小鼠肠道动力及酸敏感离子通道(ASICs)表达的影响。方法收集符合罗马Ⅳ诊断标准的IBS患儿和健康儿童的粪便制成粪菌液。构建伪无菌小鼠模型,随机分为两组:对照组予健康儿童粪菌液灌胃,实验组予IBS患儿粪菌液灌胃处理。测定小肠推进率;ELISA测定血清胃动素(MOT)和胃泌素(Gas)水平;免疫组化法检测小鼠肠道组织中ASICs表达及分布。结果与对照组比较,实验组小鼠小肠推进率及血清MOT和Gas水平均显著降低(P＜0.05);实验组小鼠小肠和结肠组织中ASIC3的表达较对照组显著增加(P＜0.05);小肠及结肠中ASIC3表达量与小肠推进率均成负相关(P＜0.05)。结论 IBS患儿的肠道菌群可促进小鼠肠道组织ASIC3表达,肠道微生物对肠道动力的影响可能与ASIC3的激活有关。

关键词: 肠易激综合征, 肠道菌群, 酸敏感离子通道, 胃肠动力

Abstract: Objective To investigate the effect of intestinal flora from children with Irritable Bowel Syndrome (IBS) on intestinal motility and acid-sensitive ion channel expression in mice. Methods Fecal samples of children with IBS identified according to Rome Ⅳcriteria and healthy children were collected and made into fecal microbiota solution. A pseudo-aseptic mouse model was established, mice were randomly divided into two groups:the control group was given fecal microbiota solution of healthy children,and the experimental group was given fecal microbiota solution of IBS children. The intestinal propulsion rate was measured, Serum Motilin (MOT) and Gastrin (Gas) were determined by ELISA, the expression and distribution of ASICs in intestinal tissues of mice were determined by immunohistochemistry. Results Compared with the control group, intestinal propulsion rate, serum MOT and Gas level were significantly reduced in the experimental group (P＜0.05), the expression of ASIC3 in the small intestine and colon of mice from experimental group was significantly increased (P＜0.05);the expression of ASIC3 in small intestine and colon was negatively correlated with the intestinal propulsion rate (P＜0.05). Conclusions Intestinal flora of children with IBS can promote the expression of ASIC3 in intestinal tissue of mice, and the effect of intestinal microorganism on intestinal motility may be related to the activation of ASICs.

Key words: irritable bowel syndrome, intestinal flora, acid-sensitive ion channels, gastrointestinal motility

中图分类号:

R392

赵军梅, 袁丽萍, 蔡洁, 李文亚, 朱克然, 桂明. 肠易激综合征患儿肠道菌群可促进小鼠肠道酸敏感离子通道蛋白的表达[J]. 基础医学与临床, 2020, 40(2): 173-177.

ZHAO Jun-mei, YUAN Li-ping, CAI Jie, LI Wen-ya, ZHU Ke-ran, Gui Ming. Intestinal flora of children with irritable bowel syndrome can promote the expression of intestinal acid-sensitive ion channel proteins in mice[J]. Basic & Clinical Medicine, 2020, 40(2): 173-177.

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