3种不同来源CD146阳性间充质干细胞亚群的生物学特性比较

基础医学与临床 ›› 2020, Vol. 40 ›› Issue (1): 41-47.

3种不同来源CD146阳性间充质干细胞亚群的生物学特性比较

王巧玲¹, 张晓彤³, 徐衍², 张乃月³, 朱榕嘉^1*, 赵春华^1*

1.中国医学科学院基础医学研究所北京协和医学院基础学院组织工程中心, 北京 100005;
2.北京大学人民医院血液科,北京 100044;
3.中国中医科学院广安门医院皮肤科,北京 100053

收稿日期:2019-10-25 修回日期:2019-11-22 出版日期:2020-01-05 发布日期:2019-12-27
通讯作者: ^*zrjia2006@126.com;zhaochunhua@vip.163.com
基金资助:
国家重点研发计划(2016YFA0101000,2016YFA0101003,2018YFA0109800);中国医学科学院医学与健康科技创新工程(2017-I2M-3-007);高等学校学科创新引智计划 (B18007)

Comparison of biological characteristics of CD146 positive subpopulations of mesenchymal stem cells derived from three different sources

WANG Qiao-ling¹, ZHANG Xiao-tong³, XU Yan², ZHANG Nai-yue³, ZHU Rong-jia^1*, ZHAO Chun-hua^1*

1. Center of Excellence in Tissue Engineering, Institute of Basic Medical Sciences CAMS,School of Basic Medicine PUMC, Beijing 100005;
2. Department of Hematology, Peking Union Medical College Hospital,Beijing 100005;
3. Department of Dermatology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China

Received:2019-10-25 Revised:2019-11-22 Online:2020-01-05 Published:2019-12-27
Contact: ^*zrjia2006@126. com;zhaochunhua@ vip.163.com

摘要/Abstract

摘要： 目的比较脐带间充质干细胞(UCMSCs)、脂肪间充质干细胞(AMSCs)以及骨髓间充质干细胞(BM-MSCs)中CD146⁺细胞亚群的生物学特性。方法用磁珠分选法分选不同组织来源的间充质干细胞,获得高纯度CD146⁺亚群;用流式细胞计量术分析表型;透射电子显微镜观察细胞结构;成脂诱导分化后油红O染色;RT-qPCR检测成脂相关基因LPL、C/EBPα和PPARγ表达;成骨诱导分化后ALP染色,检测成骨相关基因ALP、OPN和RUNX2表达;检测细胞干性基因及血管生成相关基因表达;检测细胞体外成管能力。结果 3种组织来源CD146⁺ MSCs具有相似的形态,表达除CD106外相似的细胞表面标志分子。与其他两种细胞比较,CD146⁺AMSCs表达更高的干性基因OCT-4、SOX2和NANOG。在相同的诱导时间,CD146⁺ UCMSCs成脂和成骨能力较其他两种来源的CD146⁺MSCs弱。3种组织来源的CD146⁺ MSCs均可表达血管内皮相关刺激因子BFGF、VEGF、Ang-1和EGF,但CD146⁺BM-MSCs具有更强的体外成管能力。结论 3种不同来源的CD146⁺MSC具有不同的生物学特性,为进一步研究不同组织来源的间充质干细胞的特定应用提供了基础。

关键词: 间充质干细胞, CD146, 血管生成, 生物学特性

Abstract: Objective To characterize the biological features of CD146⁺ cells from umbilical cord mesenchymal stem cells(UCMSCs), adipose-derived mesenchymal stem cells(AMSCs) and bone marrow mesenchymal stem cells(BM-MSCs). Methods MSCs from three tissues were sorted by magnetically activated cell sorting(MACS) to obtain highly purified CD146⁺ subpopulations. The phenotypes and ultra-structures of these three MSC were analyzed by flow-cytometry and transmission electron microscopy. The differentiation abilities were tested by adipogenic and osteogenic inductions. The expression of genes relative to adipogenesis, osteogenesis, stemness or angiogenesis were determined by RT-qPCR. Results CD146⁺ cells derived from three kinds of tissues had similar ultra-structures and the ability of angiogenesis. CD146⁺ AMSCs expressed highest levels of the OCT-4, SOX2 and NANOG genes.Under the same induction conditions, CD146⁺ AMSCs showed higher potential for adipogenesis and CD146⁺BM-MSCs showed higher potential for osteogenesis.However, CD146⁺ UMSCs had little ability for adipogenesis and osteogenesis. All of CD146⁺ MSCs from 3 tissues expressed BFGF, VEGF, Ang-1 and EGF although CD146⁺ BM-MSCs had stronger ability of angiogenesis in vitro. Conclusions CD146⁺ MSCs derived from the 3 sources exhibit different biological characteristics.These results provide a useful biological basis for further studies relative to application of MSCs.

Key words: mesenchymal stem cells, CD146, angiogenesis, biological characteristics

中图分类号:

王巧玲, 张晓彤, 徐衍, 张乃月, 朱榕嘉, 赵春华. 3种不同来源CD146阳性间充质干细胞亚群的生物学特性比较[J]. 基础医学与临床, 2020, 40(1): 41-47.

WANG Qiao-ling, ZHANG Xiao-tong, XU Yan, ZHANG Nai-yue, ZHU Rong-jia, ZHAO Chun-hua. Comparison of biological characteristics of CD146 positive subpopulations of mesenchymal stem cells derived from three different sources[J]. Basic & Clinical Medicine, 2020, 40(1): 41-47.

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