关键词: 关键词：HSG, 慢性阻塞性肺疾病, Wnt/PCP信号通路, 气道重建

Abstract: Objective To research the relationship between HSG and Wnt/PCP signal transduction pathway in airway remodeling in COPD，to elaborate HSG gene regulation mechanism of airway remodeling. Methods rats were divided into COPD model group and normal control group, Take small airway do fibroblasts primary cell culture, the third generation of cell lines for experiments. Used HE staining to identify COPD model, ELISA were used to detect the expression of MMP-9, PDGF and TGF-β1 in the small airways; real-time PCR were used to detect the mRNA expression of the HSG, PDGF, TGF-β1, Used Western blot to detect the expression of the HSG, RhoA protein in fibroblast; RhoA protein in fibroblast. Results model group airway fibroblasts supernatant PDGF, TGF-β1 and MMP-9 were higher normal control group(P<0.01); model group airway fibroblasts HSG protein and mRNA were lower normal control group(P<0.01); but RhoA protein and the expression of PDGF, TGF-β1, RhoA gene mRNA were higher normal control group(P<0.01); The expression of HSG was negatively correlated with that of MMP-9, PDGF, TGF-β1 and RhoA(P<0.01). Conclusions The HSG gene may regulate Wnt / PCP signaling pathway，whcih resultes in inhibiting the fibroblasts proliferation.

Key words: Key words: HSG, Chronic Obstructive Pulmonary Disease, Wnt / PCP signaling pathway, airway remodeling