基础医学与临床 ›› 2019, Vol. 39 ›› Issue (9): 1248-1251.

• 研究论文 • 上一篇    下一篇

二甲双胍对脂多糖致炎性反应小鼠的抗炎作用

刘超1,高慧婕1,杨强强2,杨方浩3,孔妮2,王宝堃3,张腾元2   

  1. 1. 济宁医学院
    2. 济宁医学院药学院
    3. 济宁医学院生物科学学院
  • 收稿日期:2018-08-15 修回日期:2018-12-07 出版日期:2019-09-05 发布日期:2019-09-06
  • 通讯作者: 高慧婕 E-mail:mianyigao@163.com
  • 基金资助:
    山东省医药卫生科技发展计划项目;济宁医学院青年教师科研扶持基金

Anti-inflammatory effect of metformin on LPS-induced inflammation in mice

  • Received:2018-08-15 Revised:2018-12-07 Online:2019-09-05 Published:2019-09-06
  • Contact: hui-jie gao E-mail:mianyigao@163.com

摘要: 目的 探究二甲双胍对脂多糖(LPS)致炎性小鼠的抗炎效应。方法 ICR小鼠分为对照组、LPS组、二甲双胍高、中和低剂量组(400, 200和100 mg/kg)。对照组和LPS组腹腔注射0.9% NaCl溶液,其余3组注射不同剂量的二甲双胍;0.5 h后,除对照组外,其余4组腹腔注射等体积的LPS致小鼠炎性反应,6 h后测量小鼠的脾指数;ELISA检测小鼠血清中炎性因子IL-6、TNF-α和IL-10的表达;RT-PCR检测小鼠肝脏组织IL-6、TNF-α和IL-10 mRNA表达的变化;HE染色检测小鼠肝脏组织炎症变化。结果 相对于LPS组,二甲双胍用药组小鼠的脾指数降低(p<0.05);外周血IL-6和TNF-α及肝脏组织中IL-6和TNF-α mRNA的表达明显下降(p <0.05);而外周血IL-10及肝脏组织中IL-10 mRNA显示上升(p <0.05);二甲双胍高剂量组可明显减轻由LPS所致小鼠肝脏的炎性反应。 结论 二甲双胍对于LPS致炎性小鼠具有一定的抗炎活性。

关键词: 二甲双胍, 炎症, 内毒素, 炎症因子

Abstract: Objective To study the anti-inflammatory effect of metformin (Met) on lipopolysaccharide (LPS)-induced inflammation in mice. Methods The mice were divided into control group, LPS group, metformin high dose group (400 mg/kg), metformin medium dose group (200 mg/kg) and metformin low dose group (100 mg/kg). The control group and LPS group were intraperitoneally injected with normal saline, the other groups were injected with different doses of metformin. After 0.5 hours, the control group was injected with normal saline, the other four groups were injected intraperitoneally with LPS. After 6 hours, the spleen index of the mice was measured. The expression of IL-6,TNF-α and IL-10 in mouse serum was detected by ELISA and the expression of IL-6,TNF-α and IL-10 mRNA in mouse liver was detected by RT-PCR. The inflammatory changes of liver tissues in each group were observed by HE staining techniques. Results Compared with the LPS group, the spleen index, IL-6 and TNF-α expression in serum and IL-6 and TNF-α mRNA expression in liver were lower in metformin group, however, IL-10 expression in serum and IL-10 mRNA expression in liver were higher in metformin group. The application of metformin(400 mg/kg) reduced the inflammatory changes of liver tissues in mouse with LPS induced inflammation. Conclusions Metformin has certain anti-inflammatory activity against inflammation induced by LPS in mice.

Key words: Metformin, inflammation, LPS, inflammatory factor

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