乳腺癌组织中JARID1B/KDM5B的表达与血液循环肿瘤细胞相关性

基础医学与临床 ›› 2018, Vol. 38 ›› Issue (4): 512-515.

乳腺癌组织中JARID1B/KDM5B的表达与血液循环肿瘤细胞相关性

赵丽华¹,位嘉²,槐英丽³,张晓敬²,张超²,林秋兰³,王克杰³,闫红燕²,郭家良²,李秋颖²

1. 北京朝阳区高井甲一号民航总医院
2. 民航总医院
3. 北京民航总医院

收稿日期:2017-11-30 修回日期:2018-01-16 出版日期:2018-04-05 发布日期:2018-03-27
通讯作者: 赵丽华 E-mail:dzhaoli@126.com
基金资助:
首都特色课题资助项目

Correlation between the expression of JARID1B/KDM5B in breast cancer tissues and the circulating tumor cells

Received:2017-11-30 Revised:2018-01-16 Online:2018-04-05 Published:2018-03-27
Contact: zhao lihua E-mail:dzhaoli@126.com
Supported by:
capital peculiarity topic assistantitem

摘要/Abstract

摘要： 目的探讨乳腺癌组织中组蛋白去甲基化酶JARID1B/KDM5B的表达及与血液循环肿瘤细胞的关系。方法：收集2014-2016年民航总医院收治的乳腺癌患者的资料，免疫组化S-P法检测癌组织JARID1B/KDM5B蛋白表达，同时用免疫荧光原位杂交法检测外周血液循环肿瘤细胞。结果发现37例受试病人中有35例确诊为浸润性乳腺癌，其中循环肿瘤细胞阳性病例(CTC数目≥2)27例，27例阳性患者中JARID1B/KDM5B免疫组化结果阴性(-)0例、(+)1例、(++)3例、(+++)23例；阴性病例(CTC数目＜2)8例，JARID1B/KDM5B免疫组化结果阴性(-)2例、(+) 2例、(++)1例、(+++)3例。乳腺癌组织中JARID1B/KDM5B的阳性表达强度与患者血液循环肿瘤细胞数量呈正相关(P＜0.05)。结论肿瘤组织JARID1B/KDM5B的表达情况对临床判断肿瘤复发、监测肿瘤转移具有一定的提示意义。

关键词: 组蛋白去甲基化酶，浸润性乳腺癌，免疫荧光原位杂交

Abstract: Objective To investigate the relation between expression of JARID1B/KDM5B in invasive breast cancer tissue and circulating tumor cells of invasive breast patients. Methods The S-P immunohistochemical method was used to observe the expression of JARID1B/KDM5B in lesions. ImFish method was used to detect circulation tumor cells. Results Twenty-seven cases of patients showed CTC positive (the number of CTC was more than or equal to 2) in 35 invasive breast cancer cases,The results of JARID1B/KDM5B expression were as follows: (-) (n = 0) and (+) in 1 case, (+ +) in 3 cases, (+ + +) in 23 cases, 8 cases of negative cases (the number of CTC < 2),JARID1B/KDM5B immune group of results: (-) (n=2), (+) in 2 cases, (+ +) in 1 case, (+++) in 3 cases，The positive relation was found in expression of JARID1B/KDM5B and circulating tumor cells（P<0.05）. Conclusions JARID1B/KDM5B positive expression in tumor tissues has a certain significance in judging the recurrence and metastasis of tumor.

Key words: JARID1B,Invasive breast cancer ,imFISH.

赵丽华位嘉槐英丽张晓敬张超林秋兰王克杰闫红燕郭家良李秋颖. 乳腺癌组织中JARID1B/KDM5B的表达与血液循环肿瘤细胞相关性[J]. 基础医学与临床, 2018, 38(4): 512-515.