关键词: 芯片, PTEN, miR-130b-3p, 膀胱癌

Abstract: Objective To further understand potential mechanism of miRNAs in bladder cancer. Methods Human microarray was used to analysis the expression of miRNAs in four pair human BC cancer tissues and adjacent normal tissues. Then RT -qPCR was used to verified that the expression of two most up-regulated miRNAs and target genes for results of miRNA/mRNA microarray. Subsequently, it was deduced that miR-130b-3p could target PTEN through a bioinformatics approach and dual-luciferase reporter assay. Next, CCK8, EDU, flow cytometry, wound healing, transwell and cytoskeleton assay were applied to prove that miR-130b could affect proliferation, apoptosis, migration and invasion of BC cells. The effects of PTEN regulated by miR-130b-3p on the key target protein expressions of PI3K/AKT and integrin β1/FAK signaling pathway were determined by western blot. Results miR-130b-3p was significantly up-regulated and negatively correlated with PTEN expression in clinical bladder cancer specimens compared with normal urothelial tissue. miR-130b-3p mimics could down-regulate PTEN expression, which leads to the activation of PI3K/AKT and integrinβ1/FAK signaling pathway related to cell proliferation, migration and invasion in bladder cancer EJ cells. When the cells were transfected with miR-130b-3p inhibitors, they could be surveyed with rearranging cytoskeleton. Conclusions The results show that miR-130b / PTEN could be used as a marker for bladder cancer.

Key words: microarray, miR-130b, PTEN, bladder cancer