关键词: 低氧, 低氧诱导因子, 静脉性溃疡, 人皮肤微血管内皮细胞

Abstract: Object to study the effects of hypoxia on the cells abilities of migration, apoptosis and expressions of gene and proteins of human dermal microvascular endothelial cells(HDMEC). Methods to culture HDMEC in hypoxia condition by putting CoCl2 in the cell incubator and all the subjects have been divided to two groups which include normal group and hypoxia group. To find the appropriate CoCl2 dose by CCK-8 experiment and the 200 μ mol /L CoCl2 is the best to establish hypoxia model. The migration ability of cells was detected by wound healing test. Alteration of apoptosis rate was detected by flow cytometry. The gene expressions were detected by RT-PCR and the protein levels were detected by Western blotting. Results The migration ability of cells was enhanced in hypoxia condition (P<0.05). The apoptosis rate was increased in hypoxia condition (P<0.05). The gene expressions of HIF-1α、HIF-1β、VEGF、iNOS were increased comparing with normal groups depending on time(P<0.05). The protein expressions of HIF-1α、VEGF、iNOS were increased comparing with normal groups depending on time(P<0.05). Conclusions Cells abilities of migration, apoptosis of HDMEC were influenced by hypoxia may have some relations with the expression of gene and protein about HIF-1α、VEGF、iNOS were increased in hypoxia condition.

Key words: hypoxia, hypoxia inducible factor-1α, venous ulcer, human dermal microvascular endothelial cells