人胚胎干细胞抑制人肝癌细胞系SK-Hep1增殖并促进凋亡

基础医学与临床 ›› 2017, Vol. 37 ›› Issue (3): 325-329.

人胚胎干细胞抑制人肝癌细胞系SK-Hep1增殖并促进凋亡

张婷,霍本念,刘洁,刘梦楠,冯涛

重庆医科大学

收稿日期:2016-08-04 修回日期:2016-11-25 出版日期:2017-03-05 发布日期:2017-02-23
通讯作者: 冯涛 E-mail:478240069@qq.com

Human embryonic stem cells inhibits the proliferation and promote apoptosis of SK-Hep human hepatoma cell line SK-Hep1

Received:2016-08-04 Revised:2016-11-25 Online:2017-03-05 Published:2017-02-23

摘要/Abstract

摘要： 目的探究在人胚胎干细胞hESCs与人肝癌细胞系SK-Hep1共培养微环境中，hESCs对SK-Hep1人肝癌细胞增殖、侵袭、迁移和凋亡的影响。方法建立hESCs与SK-Hep1人肝癌细胞系的非接触式共培养体系，将与hESCs共培养的SK-Hep1细胞作为实验组，单独培养的SK-Hep1细胞作为对照组。MTT法检测SK-Hep1细胞的增殖能力；Transwell小室法检测SK-Hep1的侵袭与迁移能力；Hoechst33258染色后观察共培养后SK-Hep1细胞核的变化；流式细胞术检测SK-Hep1细胞凋亡率。结果与hESCs共培养后，SK-Hep1细胞的增殖能力受到抑制，随着时间增加，抑制效果越显著（P <0.05）；细胞侵袭、迁移穿过Transwell小室的数量显著减少（P <0.05）；发生核固缩、变形和浓染的SK-Hep1细胞与对照组比增多；细胞凋亡比率与对照组比显著增加（P <0.05）。结论人胚胎干细胞对SK-Hep1人肝癌细胞系有抑制作用。

关键词: 胚胎干细胞, 肝癌细胞, 抑制作用

Abstract: Objective To explore effects of human embryonic stem cells ( hESCs ) onhproliferation , invasion and migration abilities of SK-Hep1 humanhepatoma cells in the co-culture micro environmen of hESCs and SK - Hep1. Methods Single cultured SK-Hep1 cells were served as control group while SK-Hep1 which non-contact co-cultured with hESCs was regarded as experimental group.The hproliferation ability of SK-Hep1 was measured by MTT method; invasion and migration ability of SK-Hep1 cells were detected by Transwell chamber method ; the nucleus variation and cell apoptosis of SK-Hep1 were detected by Hoechst33258 chromosome and flow cytometry. ResultsThe proliferation ability of SK-Hep1 cells in the experimental group was inhibited obviously compared with control group (P <0.05); the number of SK - Hep1 cells which passed through the Transwell chambers were reduced significantly compared with control group in invasion and migration experiment(P<0.05); more nucleus pycnosis and deformation appeared in experimental group than that in control group .And apoptosis rate of SK- Hep1 cells in the experimental group was significantly higher than that of in the control group (P <0.05). Conclusions Human embryonic stem cells have inhibitory effect on SK-Hep1 human hepatoma cell line.

Key words: embryonic stem cells, hepatoma cell, inhibition

中图分类号:

张婷霍本念刘洁刘梦楠冯涛. 人胚胎干细胞抑制人肝癌细胞系SK-Hep1增殖并促进凋亡[J]. 基础医学与临床, 2017, 37(3): 325-329.

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