基础医学与临床 ›› 2016, Vol. 36 ›› Issue (10): 1369-1373.

• 研究论文 • 上一篇    下一篇

乙酰葛根素对Aβ25-35诱导BV-2小鼠小胶质细胞株caspase-8 和caspase-3表达的影响

李梅,孟庆慧,蔡巧英,赵荣艳   

  1. 潍坊医学院
  • 收稿日期:2015-12-14 修回日期:2016-03-26 出版日期:2016-10-05 发布日期:2016-09-27
  • 通讯作者: 孟庆慧 E-mail:843401624@qq.com
  • 基金资助:
    山东省自然科学基金;山东省医药卫生科技发展计划项目;潍坊医学院科技创新研究基金

Effect of acetylpuerarin on expressions of caspase-8 and caspase-3 in BV-2 mousemicrogliacell lineinduced by Aβ25-35

  • Received:2015-12-14 Revised:2016-03-26 Online:2016-10-05 Published:2016-09-27

摘要: 目的观察乙酰葛根素对β淀粉样蛋白(Aβ25-35)诱导的BV-2小鼠小胶质细胞株caspase-8和caspase-3表达的影响。方法将细胞分为对照组、模型组(采用凝聚态Aβ25-35刺激BV-2小鼠小胶质细胞株建立细胞模型)、乙酰葛根素低、中、高剂量干预组及caspase-8抑制剂(Z-IETD-fmk)共6组。用Western blot检测caspase-8、caspase-3蛋白表达;实时荧光定量PCR(real-time PCR)检测caspase-8、caspase-3 mRNA表达。结果与对照组相比,模型组caspase-8、caspase-3蛋白表达明显上调(P<0.01),caspase-8、caspase-3基因表达明显增加(P<0.01);与模型组相比,乙酰葛根素各剂量组均可不同程度地明显下调caspase-8、caspase-3蛋白表达(P <0.01),明显抑制caspase-8、caspase-3基因表达(P <0.01)。结论乙酰葛根素可剂量依赖性地抑制Aβ25-35诱导BV-2小鼠小胶质细胞株caspase-8、caspase-3的表达。

关键词: 乙酰葛根素, 阿尔茨海默病, β淀粉样蛋白, 小胶质细胞, caspase

Abstract: Objective To investigate the effect of acetylpuerarin on expression of caspase-8 andcaspase-3 in BV-2 mousemicrogliacell line induced by Aβ25-35. Methods BV-2 microglia cellswere divided into six groups: control group,model group(cells were activated with condensed Aβ25-35 as cell model),acetylpuerarininterventiongroups (low-dose group,moderate-dose group, high-dose group)andcaspase-3 inhibitor (Z-IETD-fmk) group. The protein expressionof caspase-8 andcaspase-3 was detected by Westernblot. ThemRNA expressionof caspase-8 andcaspase-3 was measured by real-time PCR. ResultsCompared with control group, protein expression ofcaspase-8 and caspase-3 in model groupwas significantly up-regulated (P<0.01), and gene expression ofcaspase-8 and caspase-3was markedlyincreased (P<0.01). Compared withmodel group,protein expressionof caspase-8 andcaspase-3in acetylpuerarin each dose groupwas significantly down-regulatedin different degrees (P<0.01),and gene expressionof caspase-8 andcaspase-3 was markedlydecreased(P<0.01).ConclusionsAcetylpuerarin can inhibit caspase-8 andcaspase-3expression ofBV-2 mouse microglia cell line induced by Aβ25-35in a dose-dependent manner.

Key words: acetylpuerarin, Alzheimer’s disease, β-amyloid protein, microglia, caspase

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