关键词: PLCε, PKCα/β, TBX3, 膀胱癌, 迁移

Abstract: Objective To investigate the molecular mechanisms of PLCε in regulating the invasion and migration of human bladder cancer cells in vitro. Methods After cells treated with recombinant adenovirus, the migratory/invasive abilities of T24 cells were explored by wound healing and Transwell chamber cell migration and invasion assay; RT-PCR was used to detect the mRNA levels of PLCε; The protein levels of PLCε，PKCα，PKCβ, TBX3 and E-cadherin were determined by Western blot; QRT-PCR was used to detect the mRNA levels of TBX3 and E-cadherin .Results It was confirmed by digesting and sequencing that the recombinant adenovirus had been constructed successfully. The expression of PLCε mRNA and PLCε protein were both decreased after the infection of Ad-shPLCε. Wound healing and Transwell chamber cell migration/invasion assay showed that Ad-shPLCε treatment could inhibit the migratory and invasive activity of bladder cancer cells(P＜0.05). The results of Western blot indicated that the expression of PKCα/β in membrane was decreased(P＜0.05), and phosphorylation levels of PKCα and PKCβ were reduced. QRT-PCR and Western blot analysis demonstrated that the expression level of TBX3 was decreased, but the expression level of E-cadherin was increased. Conclusion: PLCε shRNA can inhibit migratory and invasive ability of bladder cancer cells through PKCα/β/TBX3/E-cadherin pathway.

Key words: PLCε, PKCα/β, TBX3, Bladder Cancer, migration