血栓素受体参与子痫前期的发病

基础医学与临床 ›› 2015, Vol. 35 ›› Issue (7): 921-924.

血栓素受体参与子痫前期的发病

胡继芬¹,何芳杰²,陈丽红²,吴建波¹

1. 福建医科大学附属第一医院妇产科
2. 福建医科大学附属第一医院妇产科

收稿日期:2014-08-01 修回日期:2014-12-28 出版日期:2015-07-05 发布日期:2015-06-23
通讯作者: 胡继芬 E-mail:jifenhu_fj@126.com
基金资助:
福建省教育厅科技基金项目

The Relationship Between Placental Thromboxane Receptor involved in the Pathogenesis of Preeclampsia

Received:2014-08-01 Revised:2014-12-28 Online:2015-07-05 Published:2015-06-23

摘要/Abstract

摘要： 目的探讨血栓素受体（TPr）与子痫前期发病机制的关系。方法用免疫组化和实时荧光定量PCR（Real-Time PCR）的方法检测36例子痫前期孕妇(病例组)和34例正常妊娠孕妇（对照组）胎盘中血栓素合酶(TXS)、血栓素受体(TPr)和非吞噬细胞氧化酶1 (NOX1)的表达，用酶联免疫吸附试验（ELISA）检测胎盘TXA2的表达情况。结果 TXS和TPr蛋白主要表达在细胞滋养细胞、合体滋养细胞和绒毛血管内皮细胞的胞质中，NOX1蛋白主要表达在细胞滋养细胞、合体滋养细胞、绒毛血管内皮细胞及间质细胞的胞质中。子痫前期组胎盘TXS和NOX1蛋白及mRNA表达和TXA2表达均强于对照组（P<0.05）。子痫前期TXA2表达与新生儿体重呈负相关（P<0.01）。TXA2表达与NOX1mRNA表达呈正相关（P<0.05）。结论TXA2可能通过TPr通路导致胎盘产生过量ROS并向母体血液循环释放，参与子痫前期的发病过程。

关键词: 子痫前期, 血栓素合酶, 血栓素A2, 血栓素受体, 非吞噬细胞氧化酶1

Abstract: Objective To investigate the relationship between thromboxane receptor expression and the pathogenesis of preeclampsia; Methods The expression of TXS, TPr and NOX1 in placenta of 36 preeclampsia gravidas and 34 normal gravidas were analysed with immunohistochemistry and Real-Time PCR assay. Futhermore, the expression of TXA2 was analyzed with enzyme linked immunosorbent assay. Result The expression of TXS and TPr were mainly located in the cytoplasm of placental cytotrophoblast, syncytiotrophoblast and villous vascular endothelial cells. The expression of NOX1 was mainly located in the cytoplasm of placental cytotrophoblast, syncytiotrophoblast, villous vascular endothelial cells and villous stromal cells. We found that the protein and mRNA expression of TXS and NOX1 were significantly higher in preeclamptic placentas than in normal placentas (P<0.05). Moreover, the protein expression of TXA2 were increased after preeclampsia (P<0.05). There was negative correlation between the protein expression of TXA2 and neonatal weight in preeclampsia (P<0.01). Besides, the positive correlation between the protein expression of TXA2 and NOX1mRNA in preeclampsia (P<0.05) was found. Conclusion TXA2 may improve the expression of NOX1 by activating TPr pathway which participate in the progress of preeclampsia.

Key words: Preeclampsia, Thromboxane synthase, Thromboxane A2, Thromboxane receptor, Non-phagocytic cell oxidase 1

胡继芬何芳杰陈丽红吴建波. 血栓素受体参与子痫前期的发病[J]. 基础医学与临床, 2015, 35(7): 921-924.

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