关键词: XAV939, HepG2, Wnt/β-catenin, 血管生成拟态

Abstract: Objective To investigate the impact of XAV939 to human hepatoma HepG2 cells vascular mimicry and its possible mechanism. Methods HepG2 cells were divided into control group and XAV939 treatment groups with dose of 0.5 and 1μmol/L; Tube formation of tumor cells experiment to detect the forming tubes ability of each group , RT-PCR were used to detect expressed ZEB1 and MMP-7 mRNA levels , Western blot were used to detect expression of β-catenin, ZEB1 and MMP-7 on protein levels. Results The number of vascular mimicry in the control group and the experimental group. In the control group : 9.67 ± 0.70 , 0.5μmol/L group : 5.67 ± 0.64 , 1μmol/L group : 2.27 ± 0.81,which means the number of pipeline structure formation reducing in vitro (P<0.01). Experimental group compared with the control group, ZEB1 and MMP-7 mRNA expression reduce; ZEB1, MMP-7 and β-catenin protein expression decreased(P<0.05). Conclusion XAV939 can effectively inhibition of vasculogenic mimicry formation in human hepatoma HepG2 cells.

Key words: XAV939, HepG2 , Wnt/β-catenin , vascular mimicry