bFGF对神经病理性疼痛大鼠脊髓NMDA受体表达的影响

基础医学与临床 ›› 2015, Vol. 35 ›› Issue (3): 323-328.

bFGF对神经病理性疼痛大鼠脊髓NMDA受体表达的影响

沈茜,高进,陈萍

重庆医科大学附属第一医院

收稿日期:2014-10-20 修回日期:2015-01-14 出版日期:2015-03-05 发布日期:2015-03-03
通讯作者: 陈萍 E-mail:mazuichen@163.com
基金资助:
重庆市卫生局医学科研计划项目;卫生部国家临床重点专科建设项目;重庆市医学重点学科

The effect of bFGF on the expression of NMDA receptor in spinal cord of rat with neuropathic pain

Received:2014-10-20 Revised:2015-01-14 Online:2015-03-05 Published:2015-03-03

摘要/Abstract

摘要： 目的探讨碱性成纤维细胞生长因子(bFGF)在大鼠神经病理性疼痛中的作用及对脊髓N-甲基-D-天门冬氨酸（NMDA）受体表达的影响。方法 48只雄性SD大鼠随机分为假手术组（A组）、假手术+ bFGF抗体组（B组）、SNI组（C组）、SNI+ bFGF抗体组（D组），每组12只。采用用坐骨神经分支选择性损伤(SNI)方法制备神经病理性疼痛模型。其中B组和D组于建模后1、6、9、13、16、20d鞘内注射bFGF抗体18μg，注射药物容量为40μl。A、C组则在相同时间点注射相同体积磷酸盐缓冲液（PBS）。分别于术前1d和术后1、4、7、14、21d测定机械刺激缩足反射阈值（PWMT）。术后第21d处死大鼠取L4~6节段脊髓，免疫组化测定胶质纤维酸性蛋白（GFAP）表达，RT-PCR及Westem blot检测NMDA受体亚基NR1、NR2A、和NR2B表达变化。结果　与A组和B组比较，C、D组PWMT降低，脊髓GFAP、NR1和NR2B表达升高（P<0.05）。与C组比较，D组PWMT升高，脊髓GFAP、NR1和NR2B表达降低（P<0.05）。结论鞘内注射bFGF能缓解SNI大鼠神经病理性疼痛，其机制可能与其调节NMDA受体表达有关。

关键词: 关键词：碱性成纤维细胞生长因子, NMDA受体, 神经病理性疼痛

Abstract: Objective To investigate the role of basic fibroblast growth factor (bFGF) and its effect on the expression of N-methyl-D- aspartate receptor in rat spinal cord with neuropathic pain. Methods Forty-eight male SD rats were randomly divided into four groups (n=12): sham group (group A), sham + bFGF antibody group (group B), neuropathic pain group (group C), neuropathic pain + bFGF antibody group (group D). Neuropathic pain model was produced by spared nerve injury (SNI). bFGF antibody 18 ug (40 ul) was injected intrathecally on 1, 6, 9, 13, 16 and 20 days after operation in group B and D. The equal volume of phosphate buffered saline was injected intrathecally in group A and C at the same time. The paw withdrawal mechanical threshold (PWMT) was measured on 1, 4, 7, 14 and 21 days after operation and 1 day before operation. Rats in each group were sacrificed on 21d after operation and the lumbar segments of the spinal cord (L4~6) were collected. The expression level of glial fibrillary acidic protein (GFAP) was detected by immunohistochemical analysis; The expression of N-methyl-D-aspartate receptor 1 subunit (NR1), N-methyl-D-aspartate receptor 2 subunit (NR2A or NR2B) were detected by RT-PCR and Western blot. Results Compared with group A and B, the level of PWMT was decreased and the expression of NR1 and NR2B in spinal cord was increased in group C and D (P<0.05). Compared with group C, the level of PWMT was increased and the expression of NR1 and NR2B in spinal cord was decreased in group D (P<0.05). Conclusion bFGF can reduce degree of neuropathic pain in rat with neuropathic pain induced by SNI, and the effect of bFGF on expression of NMDA receptor may associated with this phenomenon.

Key words: Key words: basic fibroblast growth factor, N-methyl-D-aspartate receptor, Neuropathic pain

沈茜高进陈萍. bFGF对神经病理性疼痛大鼠脊髓NMDA受体表达的影响[J]. 基础医学与临床, 2015, 35(3): 323-328.

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