关键词: 基质金属蛋白酶14, 白细胞介素1β, 表皮生长因子, 羊膜间充质干细胞, 细胞迁移

Abstract: Objective Research the expression regulation of epidermal growth factor (EGF) on matrix metalloproteinase 14 and interleukin-1β in the progress of human amniotic mesenchymal stem cell migration .Explore the mechanisms of EGF to promote hAMSCs migration Method Take health cesarean cultured amniotic membranes adherent mesenchymal stem cells, Using Transwell cell migration in vitro system of observation EGF on migration of hAMSCs ；By Western blotting (western blot) detection EGF action hAMSCs different time MMP14 and IL-1β protein expression changes，Using real-time quantitative PCR (Real-time PCR) detection of EGF action hAMSCs different time MMP14 and IL-1β expression levels changes of mRNA Results Primary isolated and cultured cells of human amniotic cells Identified by flow cytometry express surface marker of mesenchymal stem cells CD44 do not express CD45，Transwell chamber detect different concentration of EGF acts on hAMSCs 24h reveal that 100μg/LEGF is the best：2.0，100μg/L EGF acts on hAMSCs12h、24h and 48h，migration index namely1.6、2.0 and 1.7 ；The protein level of MMP14 and IL-1β were markedly augmentmented by 100ng/mi EGF 12h results of MMP14 are（0.81±0.20）significantly higher than control group（0.63±0.12）；the 48h results of IL-1β are（0.55±0.16）significantly higher than control group（0.30±0.05）, 100μg/L EGF upregulated the express of MMP14 and IL-1β mRNA significantly higher than control group(P＜0.05) ; Conclusion EGF have a significant role in promoting the migration of hAMSCs,in this progess EGF also upregulated the express of MMP14 and IL-1β. MMP14 IL-1β and EGF can synergistically activate the MAPK / ERK and P13K/AKT signaling pathway and MMP14 and IL-1β are involved in the regulation of EGF to promote hAMSCs migration.

Key words: matrix metalloproteinase14, interleukin 1β, epidermal growth factor, amniotic mesenchymal stem cells, cell migration