关键词: 慢性阻塞性肺疾病, 气道重塑, 气道成纤维细胞, 大鼠增殖抑制基因

Abstract: Objective Using liposome-mediated carry the eukaryotic expression vector of rats proliferation inhibition gene (rHSG) and transfected into chronic obstructive pulmonary disease (COPD) model of rat airway fibroblasts, in order to lay a basis of gene therapy that intervent the proliferation of COPD airway fibroblasts. Methods The recombinant plasmid pEGFP-rHSG were identified and sequenced.After transfected the recombinant plasmid pEGFP the-rHSG to COPD rat model of airway fibroblasts through cationic liposome and infected 24 hours, 48 hours, 3 days,5 days,7 days,detect the expression levels of rHSGmRNA on different times by real-time fluorescence quantitative polymerase chain reaction (Real time-PCR), finally,using MTT, flow cytometry detect the proliferation and apoptosis of transfected fibroblast cell.Results The recombinant plasmid pEGFP- rHSG constructed successfully.The expression of rHSGmRNA began to increase when transfected 24 hours，peak at 7 days,the differences in rHSGmRNA expression between rHSG meet each transfection group, P <0.05; After 48 hours，MTT assay show that the proliferation of airway fibroblast was significantly higher inhibited in rHSG gene transfection group than in model control group,statistically significant difference (P<0.05).After 48 hours,apoptosis were increasing by flow cytometry, compared with the model group, the difference was significant(P<0.05).Conclusion When exogenous rHSG gene transfected into the COPD rat airway fibroblasts, inhibit proliferation and induce its apoptosis.

Key words: Chronic obstructive pulmonary disease(COPD), Airway remodeling, Airway Fibroblast, rHSG gene