关键词: S100A8, Hela, 抑制

Abstract: Objective To study the effect of exogenous S100A8 on cell proliferation，apoptosis, clone formation, migration and invasion of human cervical cancer cell line Hela. Methods MTT was used to detect the cell proliferation; Hoechst staining was used to detect the cell apoptosis; Flow Cytometry was used to detect the variation of cell cycle; colony-forming assay was used to detect the ability of cell cloning formation; Wound healing assay and Transwell chamber experiment were used to detect the cell migration and invasion ability. Results 1)S100A8 inhibited cell proliferation: MTT indicated that after treatment of GST-hS100A8 for 3d, the OD value of 100 mg/L, 300 mg/L and 1000 mg/L group of GST-hS100A8 decreased by 13.64%, 19.29% and 25.06% compared with GST group, respectively (P＜0.05) .In the group of 100 mg/L, the OD value of GST-hS100A8 decreased in a time-dependent manner, especially from 72h(P＜0.05). 2)S100A8 promoted cell apoptosis: The result of Hoechst staining showed that cell apoptosis rate in GST-hS100A8 group increased by 5.18 times compared with GST group at 72h(P＜0.05). Meanwhile, the result of Flow Cytometry displayed that the peak of apoptosis of GST-hS100A8 was 19.9% at 72h, while the groups of GST and blank were no apoptotic peak. 3) We also found that the effect of GST-hS100A8 on cell cycle was not significant. 4) Colony-forming assay showed that the rate of cloning formation of GST-hS100A8 decreased by 30.2% (P＜0.05). 5) The result of wound healing assay indicated that the healing rate of GST-hS100A8 group reduced by 30.1% compared with GST group (P＜0.05) at 24h. 6) The result of Transwell indicated that the trans-membrane cell number of GST-hS100A8 group reduced by 48.9%(P＜0.05) compared with GST group at 24h. Conclusion Exogenous S100A8 could inhibit cell proliferation, cloning formation, migration, and invasion and induce cell apoptosis on human cervical cancer cell line Hela, which indicated that S100A8 could have the inhibitive effects on cervical cancer.

Key words: S100A8, Hela, Inhibitive effect