关键词: 灯盏花乙素, 核转录因子κB信号通路, 胃癌

Abstract: Objective To explore the effect of scutellarin(SCU) through regulation of nuclear transcription factor kappa B (NF-κB) p65 signaling pathway on the proliferation and migration of gastric cancer cells line MGC803. Methods To select SCUs with concentrations of 100, 250, and 500 nmol/L for subsequent experiments; MGC803 gastric cancer cells were separated into a control group, SCU-L group(100 nmol/L), SCU-M group (250 nmol/L),SCU-H group (500 nmol/L), SCU-H+PMA group(NF-κB activator, 200 nmol/L PMA). Cell plate cloning experiments were applied to detect cell proliferation. Scratch healing experiment was used to detect the migration of MGC803 cells; Tran swell assay was used to detect the invasive ability of MGC803 cells; Enzyme linked immuno-sorbent assay (ELISA) was used to detect the level of IFN-γ, IL-4, and IL-10 in the supernatant of MGC803 cells; Western blot detection of NF-κB p65 pathway related protein expression. Results SCU inhibited proliferation of MGC803 in a concentration dependent manner. SCU concentrations of 100, 250, and 500 nmol/L were selected for subsequent experiments. Compared with the control group, the number of cell clones formed, scratch healing rate, number of invasive cells, IL-10 level and p-p65/p65 ratio all decreased in SCU-L group, SCU-M group, and SCU-H group, while IFN-γ and IL-4 increased(P＜0.05). Compared with the SCU-H group, the number of cell clones, scratch healing rate, number of invasive cells, IL-10 level, and p-p65/p65 ratio obviously increased in the SCU-H+PMA group, while IFN-γ and IL-4 level significantly reduced(P＜0.05). Conclusions SCU may inhibit the proliferation and migration of gastric cancer cell line MGC803.

Key words: scutellarin, nuclear factor kappa-B signaling pathway, gastric cancer