关键词: 肾移植, 肾缺血再灌注损伤, 白藜芦醇, NF-κB信号通路

Abstract: Objective To explore the effect and mechanism of resveratrol on improving kidney injury in renal transplantation rats. Methods Forty rats were divided into sham group, model group (allogeneic kidney transplantation), high and low dose resveratrol groups [40 and 20 mg/(kg·d), intraperitoneal injection, for 7 consecutive days]. Twenty four hours after operation, the serum level of Cr, BUN, SOD and MDA was detected, the expression of Bax and Bcl-2 mRNA in renal tissues was tested by RT-qPCR, and the protein expression of IKKα, p-IκBα, and NF-κB p65 was detected by Western blot. Results Compared to the sham group, serum Cr, BUN and MDA level in the model group increased, SOD activity decreased, Bax mRNA expression in renal tissue increased, Bcl-2 mRNA expression decreased, and the expression of IKKα, p-IκBα, NF-κB p65 protein increased (P＜0.05); compared with the model group, resveratrol significantly reduced serum Cr, BUN and MDA content, while increased SOD activity, reduced Bax mRNA and IKKα, p-IκBα, NF-κB p65 protein expression, and increased Bcl-2 mRNA expression (P＜0.05). Conclusions Resveratrol has a protective effect on kidney injury in kidney transplantation rats, and the underlying mechanism is potentially related to the inhibition of activation of NF-κB signaling pathway.

Key words: kidney transplantation, renal ischemia-reperfusion injury, resveratrol, NF-κB signaling pathway