依托咪酯上调miR-290-5p减轻LPS诱导的大鼠心肌细胞系H9C2损伤

基础医学与临床 ›› 2021, Vol. 41 ›› Issue (6): 848-853.

依托咪酯上调miR-290-5p减轻LPS诱导的大鼠心肌细胞系H9C2损伤

雷家秀^*, 朱雅萍, 张雨

郑州市第七人民医院麻醉科, 河南郑州 450000

收稿日期:2020-01-02 修回日期:2020-09-15 出版日期:2021-06-05 发布日期:2021-05-31
通讯作者: ^*ijysa87@163.com
基金资助:
2018年度河南省重点研发与推广专项(182102310152)

Etomidate alleviates LPS-induced rat myocardial cell line H9C2 injury by upregulating miR-290-5p

LEI Jia-xiu^*, ZHU Ya-ping, ZHANG Yu

Department of Anesthesiology, the Seventh People's Hospital of Zhengzhou, Zhengzhou 450000, China

Received:2020-01-02 Revised:2020-09-15 Online:2021-06-05 Published:2021-05-31
Contact: ^*ijysa87@163.com

摘要/Abstract

摘要： 目的探讨依托咪酯(Eto)对脂多糖(LPS)所致大鼠心肌细胞系H9C2损伤的保护机制。方法将H9C2细胞分为对照(Co)组、LPS组(1 μg/mL的LPS处理6 h)、Eto+LPS组、miR-con+LPS组、miR-290-5p+LPS组、Eto+anti-miR-con+LPS组和Eto+anti-miR-290-5p+LPS组。四甲基偶氮唑蓝(MTT)法及流式细胞计量术分别检测细胞活力及细胞凋亡;实时荧光定量PCR(RT-qPCR)检测miR-290-5p的表达水平。酶连免疫吸附实验(ELISA)检测细胞培养液中肿瘤坏死因子α(TNF-α)和白细胞介素6(IL-6)的含量。结果与对照组比较,LPS组H9C2细胞miR-290-5p表达降低(P＜0.05),细胞存活率降低(P＜0.05),凋亡率升高(P＜0.05),TNF-α和IL-6的含量升高(P＜0.05);与LPS组比较,Eto+LPS组H9C2细胞miR-290-5p表达升高(P＜0.05),细胞存活率升高(P＜0.05),凋亡率降低(P＜0.05),细胞培养液中TNF-α和IL-6的含量降低(P＜0.05);与miR-con+LPS组比较,miR-290-5p+LPS组H9C2细胞存活率升高(P＜0.05),凋亡率降低(P＜0.05),细胞培养液中TNF-α和IL-6的含量降低(P＜0.05);与Eto+anti-miR-con+LPS组比较,Eto+anti-miR-290-5p+LPS组H9C2细胞存活率降低(P＜0.05),凋亡率升高(P＜0.05),细胞培养液中TNF-α和IL-6的含量升高(P＜0.05)。结论依托咪酯上调miR-290-5p可减轻LPS诱导的心肌细胞凋亡及炎性反应。

关键词: 依托咪酯, 脂多糖, miR-290-5p, 心肌细胞损伤, 细胞凋亡

Abstract: Objective To investigate the protective mechanism of etomidate (Eto) on lipopolysaccharide (LPS) induced myocardial injury. Methods H9C2 cells were divided into control (Co) group, LPS group (1 μg/mL LPS treatment for 6 h), Eto+LPS group, miR-con+LPS group, miR-290-5p+LPS group, Eto+anti-miR-con+LPS group, and Eto+anti-miR-290-5p+ LPS group. Tetramethylazozolium blue (MTT) method and flow cytometry were used to detect cell viability and apoptosis; real-time fluorescent quantitative PCR (RT-qPCR) was used to detect miR-290-5p expression. Enzyme-linked immunosorbent assay (ELISA) was used to detect tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) in cell culture fluid. Results Compared to the control group, the expression of miR-290-5p in H9C2 cells in the LPS group reduced (P＜0.05), the cell survival rate reduced(P＜0.05), the apoptosis rate increased (P＜0.05), and the level of TNF-α and IL-6 significantly increased (P＜0.05); compared with the LPS group, the expression of miR-290-5p in H9C2 cells in the Eto+LPS group was increased (P＜0.05), and the cell survival rate was decreased (P＜0.05), apoptotic rate was decreased (P＜0.05), TNF-α and IL-6 content were decreased (P＜0.05); compared with the miR-con+LPS group, the survival rate of H9C2 cells in the miR-290-5p+LPS group increased (P＜0.05), the apoptosis rate reduced (P＜0.05), the level of TNF-α and IL-6 reduced (P＜0.05); when compared with the Eto+anti-miR-con+LPS group, the survival rate of H9C2 cells in the Eto+anti-miR-290-5p+LPS group decreased(P＜0.05), and apoptosis rate increased (P＜0.05), the level of TNF-α and IL-6 increased(P＜0.05). Conclusions Etomidate reduces LPS-induced myocardial cell apoptosis and inflammatory response by up-regulating miR-290-5p.

Key words: etomidate, lipopolysaccharide, miR-290-5p, cardiomyocytes injury, apoptosis

中图分类号:

R542.2

雷家秀, 朱雅萍, 张雨. 依托咪酯上调miR-290-5p减轻LPS诱导的大鼠心肌细胞系H9C2损伤[J]. 基础医学与临床, 2021, 41(6): 848-853.

LEI Jia-xiu, ZHU Ya-ping, ZHANG Yu. Etomidate alleviates LPS-induced rat myocardial cell line H9C2 injury by upregulating miR-290-5p[J]. Basic & Clinical Medicine, 2021, 41(6): 848-853.

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