代谢型谷氨酸受体5(mGluR5)调控白色脂肪细胞棕色化

基础医学与临床 ›› 2021, Vol. 41 ›› Issue (1): 38-43.

代谢型谷氨酸受体5(mGluR5)调控白色脂肪细胞棕色化

罗燮¹, 曾粒², 袁磊^1*

重庆医科大学附属第二医院 1.内分泌科; 2.肾内科, 重庆 400010

收稿日期:2019-09-12 修回日期:2020-03-29 出版日期:2021-01-05 发布日期:2020-12-30
通讯作者: ^*cosmicatom@163.com
基金资助:
国家自然科学基金青年科学基金(81800771)

Metabotropic glutamate receptor 5 (mGluR5) regulates white adipocyte browning

LUO Xie¹, ZENG Li², YUAN Lei^1*

1. Department of Endocrinology; 2. Department of Nephrology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China

Received:2019-09-12 Revised:2020-03-29 Online:2021-01-05 Published:2020-12-30
Contact: ^*cosmicatom@163.com

摘要/Abstract

摘要： 目的探讨代谢型谷氨酸受体5(mGluR5)对白色脂肪细胞棕色化的调控作用。方法 Western blot检测mGluR5是否在白色及棕色脂肪组织中表达。用mGluR5拮抗剂处理分化的小鼠白色脂肪前体细胞系3T3L1,BODIPY荧光染色观察脂肪细胞脂滴形态,实时荧光定量聚合酶链反应(RT-qPCR)检测棕色脂肪细胞特异性基因及线粒体特异性基因的表达。通过细胞耗氧量(OCR)检测mGluR5拮抗剂处理后脂肪细胞呼吸能力及通过甘油释放试验检测脂肪分解能力。结果 mGluR5在白色脂肪组织及棕色脂肪组织中均表达且在白色脂肪组织中表达更高(P＜0.05)。抑制mGluR5可调节脂肪细胞脂滴形态,对棕色脂肪特异性基因及线粒体相关基因的表达、细胞呼吸、脂肪分解能力有促进的趋势,但变化差异无统计学意义。结论抑制mGluR5可促进白色脂肪细胞棕色化改变。

关键词: 代谢型谷氨酸受体5, 白色脂肪细胞, 棕色脂肪细胞, 棕色化

Abstract: Objective To investigate whether mGluR5 could regulate white adipocytes browning. Methods Western blot was used to test the expression of mGluR5 in white and brown adipose tissue. Lipid droplets morphology was microscoped after BODIPY staining. Brown adipocytes specific genes and mitochondrial related genes were analyzed by RT-qPCR in mGluR5 antagonists treated 3T3L1 cells. OCR experiments and free glycerol release assay were carried out in 3T3L1 cells after incubation with mGluR5 antagonists. Results mGluR5 expressed in both white and brown adipose tissues. The expression in white fat was higher than that in brown fat(P＜0.05). Lipid droplets morphology in adipocytes changed after treatment with mGluR5 antagonists. mGluR5 antagonists promoted the expression of browning genes and mitochondrial related genes, increased mitochondrial respiration rate and promotes lipolysis in 3T3L1 cells. Conclusions Inhibition of mGluR5 may promote white adipocyte browning.

Key words: metabotropic glutamate receptor subtype 5, white adipocyte, brown adipocyte, browning

中图分类号:

R589.9

罗燮, 曾粒, 袁磊. 代谢型谷氨酸受体5(mGluR5)调控白色脂肪细胞棕色化[J]. 基础医学与临床, 2021, 41(1): 38-43.

LUO Xie, ZENG Li, YUAN Lei. Metabotropic glutamate receptor 5 (mGluR5) regulates white adipocyte browning[J]. Basic & Clinical Medicine, 2021, 41(1): 38-43.

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