关键词: 宫颈癌, SiHa细胞, Coronin 1c, Notch/LOX/Snail通路, EMT转化

Abstract: Objective To investigate the effect of overexpression of Coronin 1c on the epithelial-mesenchymal transition(EMT) of cervical cancer cell line SiHa based on Notch1/LOX/Snail1 pathway. Methods RNA interference recombinant adenovirus targeting Coronin 1c gene(Ad-Cor-siRNA) and recombinant adenovirus containing control sequence (Ad-control) were constructed, and infected SiHa cells to silence Coronin 1c gene expression; and grouped into H8 group: H8 cells, blank control group (blank): SiHa cells not treated with any virus, Ad- Control group: SiHa cells infected with empty vector adenovirus, Ad-Cor-siRNA group: SiHa cells infected with recombinant adenovirus Ad-Cor-siRNA; Western blotting was used to detect the expression of Coronin 1c, E-cadherin, Vimentin, LOX and Snail1 in each group. The expressions of E-cadherin and Vimentin mRNA were detected by qPCR. The expressions of MMP-2, MMP-9 and Notch1 were detected by immunofluorescence assay. Results Compared with normal cervical epithelial cells H8, the expressions of Coronin 1c, Vimentin, MMP-2, MMP-9, Notch1, LOX and Snail1 proteins in SiHa cells were increased, and the expressions of E-Cadherin were decreased. After silencing of Coronin 1c, the expressions of Vimentin, MMP-2, MMP-9, Notch1, LOX and Snail1 proteins were decreased, and the expression of E-Cadherin was increased. Conclusions The overexpression of Coronin 1c protein is closely related to the EMT transformation of SiHa cells, which can activate the Notch1/LOX/Snail1 pathway and promote EMT transformation in SiHa cells.

Key words: cervical cancer, SiHa cells, Coronin 1c, Notch/LOX/Snail pathway, EMT transformation