关键词: 炎性微环境, 骨髓间充质干细胞, 肿瘤相关成纤维细胞分化

Abstract: Objective To observe whether BMSCs differentiate into TAFs in theinflammatory microenvironment simulated by IL-6 and TNF-α. Methods:The experiment was divided into 9 groups：The BMSCs group、The 50ng/ml 、100ng/ml IL-6 intervention group, The 50 ng/mL、100 ng/mL TNF-α intervention group, The 50 ng/mL IL-6+50 ng/mL TNF-α intervention group、The 50 ng/mL IL-6+100 ng/mL TNF-α intervention group、The 100 ng/mL IL-6+50 ng/mL TNF-α intervention group and The 100 ng/mL IL-6+100 ng/mL TNF-α intervention group; Continuous inducting for 42 days, detecting the changes of the BMSCs cell morphological and cycle、TAFs-tagged α-SMA and FAP protein by Phase-contrast microscope、Flow Cytometry Method and Western blot Method. Results Compared with the normal control group, The 100 ng/mL IL-6+50 ng/mL TNF-α intervention group significantly promoted BMSC cell proliferation；G1 phase decreased in proportion and S phase increased；α-SMA and FAP protein expression was significantly increased (P<0.05)；Conclusions Microenvironment simulated by the 100 ng/mL IL-6+50 ng/mL TNF-α could induce the abnormal change of the BMSCs morphological and proliferative characteristics. TAFs reference molecule α-SMA sum FAP table Proportion amount high.

Key words: Inflammatory microenvironment, BMSCs, TAFs differentiation