关键词: 关键词：选择性剪接, 长非编码RNA, PTB, 神经胶质瘤

Abstract: Objective To further investigate the role of PTB in regulating the alternative splicing of lncRNAs in a glioblastoma tumorigenesis, we performed detailed analysis for detecting and identifying alternatively spliced lncRNAs. Methods Analyzing array data and screening a specific set of alternative spliced lncRNAs. Total RNA was isolated from PTB knockdown glioblastoma cells (U87MG) or glioblastoma and normal cell lines and tissue samples, and subjected to Real-time PCR (RT–PCR) assays to detect the expression level of spliced transcripts. Alternatively spliced lncRNAs were identified as targets gene that may be regulated by PTB protein through knocking down method. Nuclear and cytoplasmic isolation were performed on T98G cells to identify cellular location of lncRNA. Results Our results uncovered PTB which impact on the transcript levels of several lncRNAs including Linc00882. Interestingly, the lncRNA linc00882 significantly exhibited a differential splicing pattern between two splice variants in the PTB-abundant glioblastoma cells. The alternative splicing transcripts were located in cell cytoplasm. Conclusions The data suggest PTB might have effect on the alternative splicing of linc00882 in glioma.

Key words: Key words：alternative splicing, long noncoding RNA, PTB, glioma