关键词: 长链非编码RNA, 幽门螺杆菌, 炎症细胞因子, 胃癌

Abstract: Objective To study the function and potential mechanism of long non-coding RNA LINC00260 in gastric cancer infected by Helicobacter pylori. Methods Quantitative real-time PCR was used to analyze the differential expression of LINC00260 in normal gastric epithelial cell GES-1 infected with HP. Gastric cancer cell line SGC-7901 was transient transfected with siRNA to down-regulate LINC00260 and co-cultured with HP, and then the inflammatory cytokines such as TNF-α, IL-1β, IL-6 and IL-8 were detected; Gastric cancer cell migration was detected by cell scratch assay after LINC00260 was knocked down. Results 1) The morphology of GES1 and SGC-7901 cells were changed after HP infection; 2) The expression level of LINC00260 was significantly reduced in gastric epithelial cells infected with HP (P<0.05); 3) The expression level of inflammatory cytokines such as TNF-α, IL-1β, IL-6 and IL-8 were down-regulated in cells co-cultured with HP following LINC00260 inhibition. 4) Knocking down the expression of LINC00260 retarded the speed of cell migration. Conclusion LINC00260 might be an oncogene which plays an important role in gastric cancer caused by HP.

Key words: Long non-coding RNA, Helicobacter pylori, inflammatory cytokine, gastric cancer