BEZ235体内外抑制人乳腺癌细胞系增殖

基础医学与临床 ›› 2016, Vol. 36 ›› Issue (3): 311-314.

BEZ235体内外抑制人乳腺癌细胞系增殖

周宪春¹,张松男²,林贞花¹,金铁峰¹

1. 延边大学基础医学院
2. 延边大学附属医院

收稿日期:2015-07-02 修回日期:2015-11-27 出版日期:2016-03-05 发布日期:2016-02-22
通讯作者: 金铁峰 E-mail:jintf@ybu.edu.cn
基金资助:
国家自然科学基金

BEZ235 inhibits the proliferation of breast cancer cells in vitro and in vivo

¹,Song-Nan ZHANGZhen-Hua Lin³,Tie-Feng Jin

Received:2015-07-02 Revised:2015-11-27 Online:2016-03-05 Published:2016-02-22
Contact: Tie-Feng Jin E-mail:jintf@ybu.edu.cn
Supported by:
National Natural Science Foundation of China

摘要/Abstract

摘要： 目的探讨BEZ235体内外抑制乳腺癌细胞的作用。方法选用人三阴乳腺癌细胞系MDA-MB-231及三阳乳腺癌细胞系MCF-7，用MTT检测细胞增殖；用裸鼠荷瘤实验分析乳腺癌细胞系体内增殖；用Western blot法检测PI3K/Akt相关信号通路蛋白的表达。结果 BEZ235对乳腺癌细胞具有明显的增殖抑制作用(p<0.01)，尤其是对三阴性乳腺MDA-MB-231细胞具有较强的体内外抑制作用(p<0.01)。结论 BEZ235可体内外抑制乳腺癌细胞，其机制可能是通过抑制PI3K/Akt信号通路蛋白的磷酸化修饰而实现的。

关键词: BEZ235, 三阴乳腺癌, 蛋白质磷酸化

Abstract: Objective To investigate the effect of BEZ235 on breast cancer cells. Methods Proliferation ability of human triple negative breast cancer cell line MDA-MB-231 and triple positive breast cancer cell line MCF-7 was detected by MTT assay; the xenograft model to analysis the ability of exhibited breast cancer proliferation in vivo; Western blot method to detect the PI3K/Akt signaling pathway related protein level. Results BEZ235 significantly inhibited the proliferation of breast cancer cells (p<0.01). Especially BEZ235 can strongly inhibited the triple negative breast cancer MDA-MB-231 cells in vivo and in vitro (p<0.01). Conclusion BEZ235 may through the inhibition of PI3K/Akt signaling pathway proteins phosphorylation to inhibition of breast cancer cells in vitro and in vivo.

Key words: BEZ235, triple negative breast cancer, protein phosphorylation

中图分类号:

R 734.2

周宪春张松男林贞花金铁峰. BEZ235体内外抑制人乳腺癌细胞系增殖[J]. 基础医学与临床, 2016, 36(3): 311-314.

Song-Nan ZHANG Zhen-Hua Lin Tie-Feng Jin. BEZ235 inhibits the proliferation of breast cancer cells in vitro and in vivo[J]. Basic & Clinical Medicine, 2016, 36(3): 311-314.