肾透明细胞癌中Eotaxin-1的表达与肿瘤增殖能力相关

基础医学与临床 ›› 2016, Vol. 36 ›› Issue (12): 1699-1702.

肾透明细胞癌中Eotaxin-1的表达与肿瘤增殖能力相关

龙浩,陈兴月,龚娟,滕燕

重庆市肿瘤研究所

收稿日期:2016-02-02 修回日期:2016-05-05 出版日期:2016-12-05 发布日期:2016-11-29
通讯作者: 龙浩 E-mail:87894277@qq.com

Eotaxin-1 is related to proliferation of clear cell renal cell carcinoma

Received:2016-02-02 Revised:2016-05-05 Online:2016-12-05 Published:2016-11-29

摘要/Abstract

摘要： 目的：探讨嗜酸性粒细胞趋化因子-1（Eotaxin-1）在肾透明细胞癌(ccRcc)中的表达情况及其与肿瘤增殖能力的关系。方法：免疫组织化学检测58例肾透明细胞癌患者肾癌组织及癌旁组织中Eotaxin-1以及增殖细胞核抗原（PCNA）的表达并分析其与肾癌临床病理特征的关系；用Western blotting法分析10例相互配对肾癌组织、癌旁组织中Eotaxin-1及PCNA的表达，并对Eotaxin-1与PCNA做相关性分析。结果：Eotaxin-1主要表达于胞浆，PCNA主要表达于细胞核内，Eotaxin-1和PCNA在肾癌组织的阳性率明显高于癌旁组织（P＜0.05）；病理分级为2-3级组的Eotaxin-1及PCNA阳性率显著高于病理分级为1级组（P＜0.01）。临床分期为Ⅲ/Ⅳ组的Eotaxin-1及PCNA阳性率显著高于临床分期Ⅰ/Ⅱ组（P＜0.05）, 有淋巴结转移组的Eotaxin-1及PCNA阳性率高于无淋巴结转移组（P＜0.05）；10 例肾癌组织中Eotaxin-1及PCNA蛋白水平显著高于癌旁组织（P＜0.01，P＜0.05）；Eotaxin-1与PCNA在肾癌组织中的表达存在相关性（P＜0.05）。结论：肾透明细胞癌组织中Eotaxin-1与肾透明细胞癌的增殖能力密切相关，对Eotaxin-1的研究可能为临床对肾透明细胞癌诊断治疗提供新的思路。

关键词: 肾透明细胞癌, Eotaxin-1, 免疫印迹

Abstract: Objective To investigate the expression of Eotaxin-1 in clear cell renal cell carcinoma(ccRCC) and its relationship with tumor proliferation. Methods The Eotaxin-1 and PCNA expression from 58 tissues of ccRCC and para carcinoma were analysised by immunohistochemistry or western blot. Results Eotaxin-1 were mainly expressed in the cytoplasm while PCNA were expressed in the cell nucleus, positive rate of Eotaxin-1 and PCNA in ccRCC tissues was significantly higher than that in para carcinoma tissues(P＜0.05), positive rate of Eotaxin-1 and PCNA in pathologic stageⅡ/Ⅲ was significantly higher than that in pathologic stageⅠ(P＜0.01). The positive rate of Eotaxin-1 and PCNA in clinical stage Ⅲ/Ⅳ was significantly higher than that in clinical stageⅠ/Ⅱ(P＜0.05). The protein level of Eotaxin-1 and PCNA in ccRCC were significantly higher than that in para-carcinoma tissues(P＜0.01，P＜0.05), Eotaxin-1 was positively related to PCNA in ccRCC tissues(P＜0.05). Conclusion Eotaxin-1 was related to proliferation in ccRCC and was expected to be a clinical indicator for ccRCC.

Key words: ccRCC, Eotaxin-1, Western blotting

龙浩陈兴月龚娟滕燕. 肾透明细胞癌中Eotaxin-1的表达与肿瘤增殖能力相关[J]. 基础医学与临床, 2016, 36(12): 1699-1702.

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