关键词: 基因芯片分析技术, 主动脉瘤, 基因表达, 荧光定量PCR

Abstract: Objective To investigate the difference of gene expression profile in aortic aneurysm and normal aorta. Methods The total RNA of 5 cases of aortic aneurysm and 4 normal aorta were isolated and cDNA was synthesized, then aRNA labeled by biotin were synthesized by in vitro transcription. The mixed probes were hybridized with Affymetrix GeneChip? Human Genome U133 Plus 2.0 Array .The fluorescent signal was scanned by Affymetrix GeneChip? Scanner 3000 .The acquired image was analyzed by GCOSvL.4 software. 6 different expression genes from profiles were validated by RT-qPCR. Results Totally 270 genes had more than two fold changed expression in aortic aneurysm, which included 211 upregulated and 59 downregulated genes. Different expressed genes were mainly involved in signal transduction, immune system process, and inflammatory response and so on. 6 genes associated with the formation of aortic aneurysms were selected for quantitative analysis, and the results of RT-qPCR were completely consistent with the results of gene microarray. Conclusions Gene differentially expressed in aortic aneurysms identified by microarray may be provide the molecular basis for the study of the pathogenesis of aortic aneurysm.

Key words: Microchip analytical procedure, Aortic aneurysm, gene expression, Real time RT-PCR