基础医学与临床 ›› 2016, Vol. 36 ›› Issue (11): 1472-1477.

• 研究论文 • 上一篇    下一篇

miR-146a调控血小板免疫活化功能

王路乔1,徐吉淋1,黄清昱1,程晓曙2,杨人强3   

  1. 1. 南昌大学第二附属医院
    2. 南昌大学第二附属医院心内科
    3. 南昌大学第二附属医院心血管内科
  • 收稿日期:2015-11-12 修回日期:2016-03-30 出版日期:2016-11-05 发布日期:2016-10-24
  • 通讯作者: 杨人强 E-mail:yangrenqiangcn@gmail.com
  • 基金资助:
    国家自然科学基金;国家自然科学基金;江西省研究生创新专项基金;江西省研究生创新专项基金

miR-146a regulates platelet immune activation

  • Received:2015-11-12 Revised:2016-03-30 Online:2016-11-05 Published:2016-10-24

摘要: 目的 探讨miR-146a对血小板免疫活化功能的影响。方法 流式细胞术检测冠心病(n=31)及健康成年人(n=35)全血中血小板PAC-1、CD62-P阳性率,q-PCR及光比浊法分别检测血浆中miR-146a及血小板聚集率;培养K562细胞,佛波酯(PMA)诱导分化为巨核细胞后,分别转染miR-146a mimics、inhibitor及其阴性对照,Western blot检测转染后细胞中TLR-4、PAC-1 及CD62-P表达水平。结果 冠心病患者miR-146a、血小板聚集率及PAC-1、CD62-P阳性率较对照组升高(P<0.05);转染miR-146a mimics后,TLR-4、PAC-1和CD62-P表达水平升高(P<0.05)。结论 miR-146a可能依赖血小板TLR-4信号通路,介导血小板免疫活化进而促进血小板聚集,加速血栓形成。

关键词: miR-146a, TLR-4, 血小板, 免疫活化

Abstract: Objective To investigate the potential roles of miR-146a in effecting on platelet immune activation. Methods Using flow cytometry to detect PAC-1 and CD62-P expression level of whole blood and q-PCR and light transmittance aggre-gometry to test miR-146a expression level and platelet aggregation rate of plasma in Coronary Artery Disease (CAD) patients (n = 31) compared with controls (n = 35). In addition, an in vitro megakaryocytic maturation and platelet formation model, as measured by CD41 and CD61 through flow cytometry, has been established by using K562 cells treated with PMA induction. The mimics, inhibitor, mimics NC and inhibitor NC of miR-146a were used to study the biological function of it. Results In CAD patients the miR-146a, PAC-1 and CD62-P positive expression rate and platelet aggregation rate were increased (P<0.05) compared with controls. In addition, miR-146a leaded to enhance TLR-4, PAC-1 and CD62-P expression level (P<0.05) when challenged with miR-146a mimics. Conclusion This study has demonstrated that miR-146a can affects the platelet immune activation via TLR-s signal pathway, which imply that miR-146a might be able to act as novel tools for regulation of platelet activation.

Key words: miR-146a, TLR-4, platelet, immune activation

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