基础医学与临床 ›› 2015, Vol. 35 ›› Issue (7): 867-872.

• 研究论文 •    下一篇

光动力疗法对子宫内膜癌Ishikawa细胞系细胞周期和凋亡的影响

宗丽菊1,王颉1,刘洪丽1,冯进波1,张友忠2   

  1. 1. 山东大学齐鲁医院
    2. 山东大学齐鲁医院妇产科
  • 收稿日期:2014-10-24 修回日期:2015-01-21 出版日期:2015-07-05 发布日期:2015-06-23
  • 通讯作者: 张友忠 E-mail:zhangyouzhong@vip.sina.com
  • 基金资助:
    国家自然科学基金资助项目

Effects of photodynamic therapy on cell cycle and apoptosis in endometrial cancer Ishikawa cell line

  • Received:2014-10-24 Revised:2015-01-21 Online:2015-07-05 Published:2015-06-23

摘要: 目的 探讨光动力疗法PDT对子宫内膜癌Ishikawa细胞系细胞周期及凋亡的影响。方法 以四甲基吡啶卟啉TMPyP为光敏剂的PDT处理Ishikawa细胞,显微镜观察细胞形态;细胞计数试剂盒CCK-8检测细胞的存活率;流式细胞术检测细胞周期;使用Annexin V-FITC/PI凋亡检测试剂盒及流式细胞术检测细胞的凋亡;蛋白免疫印迹法检测Bcl-2、NF-κB P65及磷酸化NF-κB P65的表达。结果 PDT可以改变Ishikawa细胞的形态;降低细胞存活率(P<0.05),并与光敏剂TMPyP的浓度和激光能量密度有关;PDT引起细胞S期阻滞(P<0.05);诱导Ishikawa细胞发生凋亡(P<0.05);降低 Bcl-2蛋白的表达和NF-κB P65的磷酸化水平(P<0.05)。结论 PDT可能通过下调Bcl-2、NF-κB的活性引起Ishikawa细胞S期阻滞和细胞凋亡。

关键词: 关键词:子宫内膜癌Ishikawa细胞, 光动力疗法, 细胞周期, 细胞凋亡

Abstract: Objective To investigate the effects of photodynamic therapy(PDT) on cell cycle and apoptosis in endometrial cancer Ishikawa cell line. Methods Ishikawa cell were treated by PDT with photosensitizer 5,10,15,20-tetrakis(1-methylpyridinium-4-yl)porphyrin(TMPyP). Cell morphology were observed by inverted microscope. Survival rate of Ishikawa cell was evaluated with Cell Counting Kit-8 (CCK-8). Cell cycle was analyzed by flow cytometry (FCM). Cell apoptosis was analyzed with Annexin V-FITC/PI Apoptosis Kit for FCM. The expression of Bcl-2、NF-κB P65 and phospho-NF-κB P65(p-NF-κB P65) and were detected by Western blotting. Results PDT could cause Ishikawa cell morphological changes. Survival rate decreased in Ishikawa cell treated by PDT after 24h(P<0.05) and the rate was related with photosensitizer concentration and energy intensity. FCM analysis indicated PDT could induce S phase arrest and apoptosis(P<0.05). The expression of Bcl-2 protein and phosphorylation of NF-κB P65 were down regulated in Ishikawa cell after PDT(P<0.05). Conclusion PDT could induce S phase arrest and apoptosis in endometrial cancer Ishikawa cell , the possible mechanism is down-regulation of Bcl-2 and NF-κB.

Key words: Key words: endometrial cancer Ishikawa cell, photodynamic therapy, cell cycle, cell apoptosis

中图分类号: