干扰SULT2B1抑制人肝癌细胞BEL-7402的上皮间质化

基础医学与临床 ›› 2015, Vol. 35 ›› Issue (11): 1503-1507.

干扰SULT2B1抑制人肝癌细胞BEL-7402的上皮间质化

杨晓明¹,李桂忠¹,田珏¹,徐华¹,杨晓玲¹,殷莲华²

1. 宁夏医科大学
2. 复旦大学

收稿日期:2015-04-28 修回日期:2015-07-01 出版日期:2015-11-05 发布日期:2015-11-03
通讯作者: 杨晓明 E-mail:yxming1999@163.com
基金资助:
SULT2B1调控上皮-间质转换肝癌细胞侵袭与迁移过程中的作用及机制研究

SULT2B1 interference inhibits the epithelial-mesenchymal transition of human hepatocellular carcinoma BEL-7402 cells

Received:2015-04-28 Revised:2015-07-01 Online:2015-11-05 Published:2015-11-03
Contact: Xiao-Ming YANG E-mail:yxming1999@163.com

摘要/Abstract

摘要： 目的研究羟基类固醇硫酸基转移酶SULT2B1与人肝癌细胞BEL-7402上皮间质转化的关系及其作用机制。方法细胞分为空白对照组（NC）、阴性对照组（control-siRNA）和SULT2B1干扰组（SULT2B1-siRNA），LipofectamineTM 2000脂质体转染细胞，Real-time PCR和Western blot检测干扰效果。Western blot检测紧密连接蛋白（ZO-1）、波形蛋白（vimentin）和转录因子ZEB1的表达。结果 SULT2B1干扰BEL-7402细胞后其mRNA与蛋白表达均显著降低（P<0.05），ZO-1表达水平升高（P<0.05），vimentin的表达及ZEB1的蛋白水平均降低（P<0.05）。结论干扰SULT2B1通过下调ZEB1的表达抑制人肝癌细胞BEL-7402的上皮间质化。

关键词: 关键词：肝癌细胞, 羟基类固醇硫酸基转移酶SULT2B1, 上皮间质转化, ZEB1

Abstract: Objective To investigate the relationship of hydroxysteroid sulfotransferase 2B1 (SULT2B1) and epithelial-mesenchymal transition (EMT), exploring its possible mechanisms. Methods cells were divided into three groups: control group, negative control siRNA group and SULT2B1-siRNA group. LipofectamineTM 2000 was employed for transient transfection. The SULT2B1 interference efficiency in treated BEL-7402 cells was detected by real-time PCR and western blot assays respectively. The protein levels of zonula occludens-1 (ZO-1), vimentin and transcriptional factor ZEB1 in BEL-7402 cells with SULT2B1 interference were determined by western blot analysis. Results The mRNA and protein levels of SULT2B1 in treated BEL-7402 cells were decreased significantly as compared with the negative control group (P< 0.05). The protein level of ZO-1 was up-regulated significantly (P<0.05), while vimentin and the transcriptional factor ZEB1 were decreased in BEL-7402 cells with SULT2B1 interference (P<0.05),. Conclusion SULT2B1 interference can inhibit the epithelial-mesenchymal transition of BEL-7402 cells by down-regulating transcriptional factor ZEB1.

Key words: Key words: hepatocellular carcinoma, hydroxysteroid sulfotransferases 2B1, epithelial- mesenchymal transition, ZEB1

中图分类号:

R735.7

杨晓明李桂忠田珏徐华杨晓玲殷莲华. 干扰SULT2B1抑制人肝癌细胞BEL-7402的上皮间质化[J]. 基础医学与临床, 2015, 35(11): 1503-1507.

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