关键词: 胃癌, 血清应答因子（SRF）, Y-27632（ROCK通路阻断剂）, 胃癌SGC-7901细胞株, RNA干扰

Abstract: Objective To study the effect of Y-27632 on invasion and motility of SGC-7901 gastric carcinoma cells, and discuss whether Y-27632 excerts the effect by attenuating SRF expression. Methods SGC-7901 gastric carcinoma cells were divided into 3 groups: 1) blank control group; 2) Y-27632 group; 3) siRNA-SRF-1107 group. Transfected siRNA-SRF or incubated by Y-27632 48h. The effect of Y-27632 on proliferation suppressions of SGC-7901 gastric carcinoma cells was detected by CCK-8 assay. Cell invasion was analysed by Transwell and wound healing test. The expressions of SRF, ROCK1, E-cadherin, β-catenin, F-actin, MRTF-A and Cyclin D1 were detected by Western blot. Results Y-27632 inhibited invasion （P<0.05）and had no effect on proliferation of SGC-7901 gastric carcinoma cells（P>0.05）. Western blot showed that are treated with Y-27632, the ROCK1, MRTF-A, F-actin, SRF protein expressions were reduced by 37.0%, 44.3%, 62.7% and 62.7% respectively, and E-cadherin protein expression was up-regulated by 2.64 folds（P<0.05）.Conclusion The inhibition of ROCK and up-regulation of E-cadherin by Y-27632 can inhibit the invasion and migration of SGC-7901 gastric carcinoma cells that is done at least, in part, by attenuating SRF expression.

Key words: Gastric carcinoma, serum response factor(SRF), Y-27632(ROCK inhibitor), SGC-7901 cell line, RNA interference