关键词: 表皮生长因子, 去势抵抗性前列腺癌, 雄激素受体, 酪氨酸激酶, 磷酸化

Abstract: Objective To investigate the influence of epidermal growth factor on the proliferation and androgen receptor phosphorylation of prostate cancer cells at the absence of androgen. Methods LNCaP and LAPC4 AR were used for the study, after EGF treatment,western-blot was used to detect AR phosphorylation in LNCaP and LAPC4 cells; Knockdown of Src and Ack1 genes with siRNA transfection to observe the effect on androgen receptor phosphorylation; Cell Counting Kit-8(CCK-8) was used to measure the cell proliferation; Quantitive RT-PCR was used to analyze the expression of prostate–specific antigen and human kallikrein-2 mRNA. Results EGF can induce AR specific site phosphorylation at Try-534 and Try-267 by Src kinase and another unknown kinase; Compared with untreated control, EGF improved the proliferation of prostate cancer cells（p<0.05） and increased the expression of prostate –specific antigen（p<0.05） and human kallikrein-2（p<0.05） Conclusions EGF can induce proliferation and AR phosphorylation of prostate cancer by intracellular non-receptor tyrosine kinases.

Key words: Epidermal growth factor, Castration-resistant prostate cancer, Androgen receptor, Tyrosine kinase, Phosphorylation