慢性乙型肝炎患者外周血单个核细胞Toll样受体3的表达降低

基础医学与临床 ›› 2013, Vol. 33 ›› Issue (8): 1028-1031.

慢性乙型肝炎患者外周血单个核细胞Toll样受体3的表达降低

周兰英¹,蒋乐龙¹,李定梅¹,阎青¹,于红缨²,姚志军²,向开敏³

1. 怀化医学高等专科学校
2. 怀化市第一人民医院
3. 中南大学湘雅三医院

收稿日期:2012-05-29 修回日期:2012-11-02 出版日期:2013-08-05 发布日期:2013-07-18
通讯作者: 周兰英 E-mail:16215092@qq.com

Reduced expression of Toll-like receptors 3 in peripheral blood mononuclear cells of chronic hepatitis B patients

Received:2012-05-29 Revised:2012-11-02 Online:2013-08-05 Published:2013-07-18

摘要/Abstract

摘要： 目的探讨慢性乙型肝炎患者外周血单个核细胞Toll样受体3（TLR3）的表达及其临床意义。方法分别采集慢性乙型肝炎患者和健康志愿者外周血，荧光定量PCR法检测血清HBV DNA复制水平；使用RT-PCR、流式细胞术以及免疫印迹技术分别检测外周血单个核细胞TLR3的mRNA、蛋白的表达；使用ELISA法检测血清中肿瘤坏死因子α（TNF-α）和干扰素β（IFN-β）水平。结果慢性乙型肝炎患者外周血单个核细胞中的TLR3表达显著低于健康志愿者，且降低水平与血清HBV DNA复制水平相关；慢性乙型肝炎患者外周血TNF-α、IFN-β浓度显著低于健康志愿者，且降低的水平与血清HBV DNA复制水平相关。结论慢性乙型肝炎患者外周血单个核细胞TLR3的表达与乙肝病毒的复制水平相关。

关键词: 关键词：慢性乙型肝炎, Toll样受体3, 外周血单个核细胞

Abstract: Objective To investigate the Toll-like receptor 3 (TLR3) expressions in peripheral blood mononuclear cells of chronic hepatitis B patients and explore its clinical significance. Methods The peripheral blood were collected from chronic hepatitis B patients and healthy volunteers, serum HBV DNA replication levels were detected by quantitative PCR; TLR3 mRNA and protein expression levels in peripheral blood mononuclear cells were detected by RT-PCR, flow cytometry and Western blot respectively; the serum levels of TNF-α and IFN-β were measured by ELISA. Results The TLR3 expression in peripheral blood mononuclear cells of chronic hepatitis B patients was significantly lower than that in healthy volunteers and related to the level of serum HBV DNA replication; the serum TNF-α，IFN-β concentrations of chronic hepatitis B patients were significantly lower than that in healthy volunteers and related to the level of serum HBV DNA replication level. Conclusion The TLR3 expression in peripheral blood mononuclear cells of chronic hepatitis B patients was associated with the proliferation of hepatitis B virus.

Key words: Key words: chronic hepatitis B, Toll-like receptors 3, peripheral blood mononuclear cells

中图分类号:

R512.6+2

周兰英蒋乐龙李定梅阎青于红缨姚志军向开敏. 慢性乙型肝炎患者外周血单个核细胞Toll样受体3的表达降低[J]. 基础医学与临床, 2013, 33(8): 1028-1031.

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