人单核细胞株THP-1中HMGN2的分离纯化及其抗菌活性

基础医学与临床 ›› 2013, Vol. 33 ›› Issue (2): 156-160.

人单核细胞株THP-1中HMGN2的分离纯化及其抗菌活性

张敏¹,冯云²,³

1. 四川省医学科学院·四川省人民医院
2.
3. 四川大学口腔疾病研究国家重点实验室

收稿日期:2010-11-22 修回日期:2012-04-17 出版日期:2013-02-05 发布日期:2013-01-25
通讯作者: 张敏 E-mail:zhangmin_0924@qq.com

Isolation and Purification of HMGN2 from Human Monocyte Cell line THP-1 and its antimicrobial activity

Received:2010-11-22 Revised:2012-04-17 Online:2013-02-05 Published:2013-01-25

摘要/Abstract

摘要： 目的从人单核细胞株THP-1中分离与纯化HMGN2抗菌肽，应用微量琼脂糖弥散法检测抗菌活性，以探讨HMGN2分子的抗菌作用。方法采用HPLC分离纯化, 琼脂糖弥散法筛选纯化有抗菌活性的组分，用Tricine-SDS-PAGE电泳初步测定其分子质量，以质谱法进行分子质量的精确分析。结果从人THP-1细胞中分离纯化出HMGN2抗菌肽, 琼脂糖弥散法检测显示其对大肠杆菌氨苄青霉素耐药株ML-35p有较强的抑菌活性，对大肠杆菌标准株ATCC25922和临床分离株54080亦有较强的抗菌活性，而对金黄色葡萄球菌ATCC25923未检测出抗菌活性。结论：HMGN2是一种抗菌分子，可能参与了体内的天然免疫防御作用。

关键词: 人THP-1细胞, HMGN2, 抗菌肽, 分离纯化

Abstract: Objective To isolate and purify antimicrobial polypeptides HMGN2 from human monocyte THP-1 and detect its antimicrobial activity of HMGN2 by agar diffusion test. Methods: HMGN2 antimicrobial polypeptide were purified by the Reverse Phase HPLC and HMGN2 molecular weight were detected by Tricine-SDS-PAGE and the mass spectrum. The antimicrobial activity of HMGN2 was detected by agar diffusion test. Results HMGN2 had potent antimicrobial activity against E.coli ML-35p, some extent against E.Coli ATCC 25922 and clinical isolate 54080. However, its was inactive against S.aureus ATCC25923. Conclusion HMGN2 protein is an antimicrobial molecule and it is probably involved in the defensive of the innate immunity.

Key words: Human THP-1 cells, HMGN2, Antimicrobial polypeptide, Isolation and Purification

张敏冯云. 人单核细胞株THP-1中HMGN2的分离纯化及其抗菌活性[J]. 基础医学与临床, 2013, 33(2): 156-160.

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