关键词: 宫颈鳞癌, 宫颈腺癌, Hela, Siha, TPX2, RT-pcr, 免疫组织化学

Abstract: [ABSTRACT] Objective:To study the expression of TPX2 protein and mRNA in human cervical cancer and the relationship between its expression and clinicopathologyical features of cervical cancer.Methods:Immunohistochemistry、reverse transcription polymerase chain reaction were used to analyze the expression levels of TPX2 mRNA and protein respectively, in fresh invasive cervical squamous cancer (n=62)、cervical adenocarcinoma（n=17） normal cervical uteri (n=15) ,CIN tissues(n=30), squamous carcinoma of the cervix Siha cell lines and adenocarcinoma of the uterine cervix Hela cells，①By RT-PCR, there was no expression of TPX2 mRNA in normal cervical tissues, The expression of TPX2 mRNA cervical squamous carcinoma is 0.441 ± 0.011, cervical adenocarcinoma is 0.467±0.031. Hela cell lines is 1.923±0.04,Siha cell lines is 1.679±0.042，There was significant difference between them and normal cervical tissues, (P<0.05)②By immunohistochemistry, normal cervical tissues did not express TPX2. The expression of TPX2 in CIN and cervical carcinoma were increased respectively ,There was significant difference between the three tissues (P<0.05). normal cervical glandular epithelium tissues did not express TPX2，The expression of TPX2 in adenocarcinoma of the uterine cervix is strong positive, There was significant difference between the two tissues (P<0.01).③The expression of TPX2 were correlated with histologicalgrade、 FIGO stages、 and lymph nodemetastasis (P<0. 05) and not correlated with age (P>0.05) 。④The expression of TPX2 in cervical gland cancer Hela cell line is stronger than squamous cancer Siha cell line. Conclusion:There is no expression of TPX2 in normal cervical tissues. TPX2 is probably involved in carcinogenesis and development of cervical carcinoma.The detection of TPX2 expression may play an important role in early diagnosis and evaluation of prognosis in patients with cervical carcinoma.The high-expression of TPX2 might become a novel biomarker for studying them echanism underlying the tumorigenesis and lymphaticmetastasis of cervical cancer and therapeutics target point for cervical cancers.

Key words: cervical squamous carcinoma, cervical adenocarcinoma, Hela, Siha , TPX2, RT-pcr, immunohistochemistry