基础医学与临床 ›› 2012, Vol. 32 ›› Issue (2): 186-190.

• 研究论文 • 上一篇    下一篇

9-顺式维甲酸对甲状腺鳞癌细胞周期及CyclinD1、CDK4表达的影响

张秀梅,肖建英,刘超   

  1. 辽宁医学院
  • 收稿日期:2011-06-30 修回日期:2011-11-14 出版日期:2012-02-05 发布日期:2012-01-12
  • 通讯作者: 肖建英 E-mail:xiaojianying6886@yahoo.com.cn

Effects of 9-cis-retinoic acid on cell cycle and expressions of CyclinD1 and CDK4 in thyroid squamous cell carcinoma cell line SW579

  • Received:2011-06-30 Revised:2011-11-14 Online:2012-02-05 Published:2012-01-12

摘要: 摘 要:目的 探讨9-顺式维甲酸(9-cisRA)对甲状腺鳞癌细胞株SW579细胞周期及周期因子表达的影响。方法以终浓度分别为为10-7 mol/L、10-6 mol/L、5×10-6 mol/L、10-5 mol/L 的9-cisRA处理SW579细胞株,采用MTT比色法测定细胞增殖活性;流式细胞仪检测细胞周期;用RT-PCR方法检测SW579细胞CDK4、CyclinD1mRNA的表达水平;用Western blotting检测SW579细胞CDK4、CyclinD1的蛋白表达。结果 9-cisRA作用后,MTT结果显示,各组细胞均出现显著的生长抑制作用,生长抑制率明显升高,并呈剂量依赖性。流式细胞仪分析,各组细胞均随着药物浓度的升高,S期细胞在细胞周期中所占比例逐渐减少,G1期细胞则明显增加(P<0.01),细胞滞留在G1期。RT-PCR检测结果表明,经不同浓度9-cisRA处理的细胞CDK4 mRNA表达水平没有明显变化;CyclinD1的表达水平明显下调;Western blotting检测结果表明经不同浓度9-cisRA处理的细胞CDK4蛋白表达水平没有明显变化,CyclinD1蛋白表达水平明显下降。结论9-cisRA对SW579细胞有显著的生长抑制作用,可能与抑制CyclinD1的表达,从而将细胞阻滞于G1期有关。

关键词: 关键词:9-顺式维甲酸, SW579, 细胞周期, CDK4, CyclinD1

Abstract: Abstract:Objective To study the effects of 9-cis retinoic acid(9-cisRA) on cell cycle and expressions of CyclinD1 and CDK44 in thyroid squamous cell carcinoma cells(SW579).Methods Treated with different dose of 9-cisRA (final concentration of 10-7 mol/L、10-6 mol/L、5×10-6mol/L、10-5mol/L). the proliferation of the cells was observed with MTT and the cell cycle was measured by flow cytometry;the CDK4 and CyclinD1 mRNA expression levels were determined by RT-PCR;CDK4 and CyclinD1 protein expression were determined by western blotting. Results After cells were treated with 9-cisRA the proliferation index decreased markedly with a dose-dependent manner.Flow cytometry analysis showed the percent of G1 phase cells was significantly higher than that of the control groups and the percent of S phase cells was lower than that of the control groups .The cell cycle was arrested at G1 stage;The mRNA expression of the CDK4 was not increased; the mRNA expression of CyclinD1 was. descreased dramatically; Distinct changes in the expressions of CDK4 protein was not observed; the expressions of CyclinD1 protein was. descreased dramatically; Conclusions 9-cisRA inhibited the proliferation of SW579 cell in a dose-dependent manner,It was related with down-regulation of CyclinD1 and arresting sw579cell at G1 phase.

Key words: Key words: 9-cis- retinoic acid, SW579, cell cycle, CyclinD1, CDK4

中图分类号: